| |  Online-Ressource |
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| Verfasst von: | Weichsel, Julian [VerfasserIn]  |
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| | Herold, Nikolas [VerfasserIn] |
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| | Lehmann, Maik J. [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| | Schwarz, Ulrich S. [VerfasserIn] |
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| Titel: | A quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopy |
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| Verf.angabe: | Julian Weichsel, Nikolas Herold, Maik J. Lehmann, Hans-Georg Kräusslich, Ulrich S. Schwarz |
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| Jahr: | 2010 |
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| Umfang: | 12 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online veröffentlicht am 6. November 2009 ; Gesehen am 18.10.2023 |
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| Titel Quelle: | Enthalten in: Cytometry / A |
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| Ort Quelle: | Hoboken, NJ : Wiley-Liss, 2003 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 77A(2010), 1 vom: Jan., Seite 52-63 |
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| ISSN Quelle: | 1552-4930 |
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| Abstract: | The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the perspective to clarify such an issue by processing many samples in a high-throughput manner. To analyze the alterations in the actin cytoskeleton within an automated setting, large-scale image acquisition and analysis were established for JC-53 cells stained for actin. As a quantitative measure in such an automated approach, we suggest a parameter called image coherency. We successfully benchmarked our analysis by calculating coherency for both a biophysical model of the actin cytoskeleton and for cells whose actin architecture had been disturbed pharmacologically by latrunculin B or cytochalasin D. We then tested the influence of HIV-1 infection on actin coherency, but observed no significant differences between uninfected and infected cells. © 2009 International Society for Advancement of Cytometry |
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| DOI: | doi:10.1002/cyto.a.20818 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1002/cyto.a.20818 |
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| | kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20818 |
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| | DOI: https://doi.org/10.1002/cyto.a.20818 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | actin cytoskeleton |
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| | biophysical modeling |
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| | high-throughput microscopy |
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| | HIV-infection |
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| | image analysis |
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| K10plus-PPN: | 1504895398 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopy / Weichsel, Julian [VerfasserIn]; 2010 (Online-Ressource)
