| Online-Ressource |
Verfasst von: | Lahdou, Imad [VerfasserIn]  |
| Engler, Christoph [VerfasserIn]  |
| Mehrle, Stefan [VerfasserIn]  |
| Daniel, Volker [VerfasserIn]  |
| Sadeghi, Mahmoud [VerfasserIn]  |
| Opelz, Gerhard [VerfasserIn]  |
| Terness, Peter [VerfasserIn]  |
Titel: | Role of human corneal endothelial cells in T-cell-mediated alloimmune attack in vitro |
Verf.angabe: | Imad Lahdou, Christoph Engler, Stefan Mehrle, Volker Daniel, Mahmoud Sadeghi, Gerhard Opelz, and Peter Terness |
E-Jahr: | 2014 |
Jahr: | March 2014 |
Umfang: | 9 S. |
Fussnoten: | Gesehen am 21.09.2021 |
Titel Quelle: | Enthalten in: Investigative ophthalmology & visual science |
Ort Quelle: | Rockville, Md. : ARVO, 1977 |
Jahr Quelle: | 2014 |
Band/Heft Quelle: | 55(2014), 3 vom: März, Seite 1213-1221 |
ISSN Quelle: | 1552-5783 |
Abstract: | Human corneal endothelial cells (HCEC) are a potential target of immune attack after corneal transplantation. The aim of this in vitro study was to investigate the role of HCEC during the alloimmune response of T-cells by examining cytokine profiles, function of the immunosuppressive enzyme indoleamine 2,3-dioxigenase (IDO), major histocompatibility complex (MHC-I/-II), T-cell proliferation, and the induction of cell death. Real-time PCR and RP-HPLC were used to determine IDO expression and activity. Multiplex assay was performed for quantification of cytokine levels. T-cell proliferation was assessed by thymidine incorporation, and HCEC cell death was measured by flow cytometry. Human corneal endothelial cells induce strong proliferation of allogeneic T-cells and an increase of proinflammatory cytokines such as interleukin-1α (IL-1α), IL-1β, IL-6, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α). Tumor necrosis factor-alpha (and to a lesser extent IFN-γ) induces apoptosis. Moreover, IFN-γ strongly upregulates MHC-II molecules and IDO activity in HCEC as reflected by high kynurenine (Kyn) concentrations. Interestingly, the T-cell response was not affected by increased IDO activity, since blocking of IDO did not affect the proliferation rate. Indoleamine 2,3-dioxigenase-induced Kyn levels did not exceed concentrations of 175 ± 20 μM. Concentrations of ≥400 μM Kyn were required to suppress T-cell proliferation. Our data show that T-cell attack on HCEC leads to increased concentrations of proinflammatory cytokines. Inflammatory cytokines induce apoptosis and upregulate MHC-II molecules and IDO in HCEC. Although increased IDO activity does not influence the T-cell response, it constitutes an inflammatory marker of the alloimmune response toward HCEC. |
DOI: | doi:10.1167/iovs.13-11930 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag ; Resolving-System: https://doi.org/10.1167/iovs.13-11930 |
| kostenfrei: Volltext: http://dx.doi.org/10.1167/iovs.13-11930 |
| DOI: https://doi.org/10.1167/iovs.13-11930 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1525061453 |
Verknüpfungen: | → Zeitschrift |
Role of human corneal endothelial cells in T-cell-mediated alloimmune attack in vitro / Lahdou, Imad [VerfasserIn]; March 2014 (Online-Ressource)