Improved synthesis of N-Benzylaminoferrocene-based prodrugs and evaluation of their toxicity and antileukemic activity

• Verfasst von: Daum, Steffen [VerfasserIn]
• Verfasst von: Sellner, Leopold [VerfasserIn]
• Verfasst von: Zenz, Thorsten [VerfasserIn]
• Titel: Improved synthesis of N-Benzylaminoferrocene-based prodrugs and evaluation of their toxicity and antileukemic activity
• Verf.angabe: Steffen Daum, Vasiliy F. Chekhun, Igor N. Todor, Natalia Yu. Lukianova, Yulia V. Shvets, Leopold Sellner, Kerstin Putzker, Joe Lewis, Thorsten Zenz, Inge A.M. de Graaf, Geny M.M. Groothuis, Angela Casini, Oleksii Zozulia, Frank Hampel, and Andriy Mokhir
• E-Jahr: 2015
• Jahr: January 29, 2015
• Umfang: 10 S.
• Fussnoten: Gesehen am 30.01.2017
• Titel Quelle: Enthalten in: Journal of medicinal chemistry
• Ort Quelle: Washington, DC : ACS, 1959
• Jahr Quelle: 2015
• Band/Heft Quelle: 58(2015), 4, Seite 2015-2024
• ISSN Quelle: 1520-4804
• DOI: doi:10.1021/jm5019548
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1552536017

Abstract

We report on an improved method of synthesis of N-benzylaminoferrocene-based prodrugs and demonstrate its applicability by preparing nine new aminoferrocenes. Their effect on the viability of selected cancer cells having different p53 status was studied. The obtained data are in agreement with the hypothesis that the toxicity of aminoferrocenes is not dependent upon p53 status. Subsequently the toxicity of a selected prodrug (4) was investigated ex vivo using rat precision cut liver slices and in vivo on hybrid male mice BDF1. In both experiments no toxicity was observed: ex vivo, up to 10 μM; in vivo, up to 6 mg/kg. Finally, prodrug 4 was shown to extend the survival of BDF1 mice carrying L1210 leukemia from 13.7 ± 0.6 days to 17.5 ± 0.7 days when injected daily 6 times at a dose of 26 μg/kg starting from the second day after injection of L1210 cells.