Generation of Se-fortified broccoli as functional food

• Verfasst von: Hsu, Fu-Chen [VerfasserIn]
• Verfasst von: Wirtz, Markus [VerfasserIn]
• Verfasst von: Heppel, Simon [VerfasserIn]
• Verfasst von: Bogs, Jochen [VerfasserIn]
• Verfasst von: Krämer, Ute [VerfasserIn]
• Verfasst von: Khan, Muhammad Sayyar [VerfasserIn]
• Verfasst von: Hell, Rüdiger [VerfasserIn]
• Verfasst von: Rausch, Thomas [VerfasserIn]
• Titel: Generation of Se-fortified broccoli as functional food
• Titelzusatz: impact of Se fertilization on S metabolism
• Verf.angabe: Fu-Chen Hsu, Markus Wirtz, Simon C. Heppel, Jochen Bogs, Ute Krämer, Muhammad Sayyar Khan, Achim Bub, Rüdiger Hell and Thomas Rausch
• E-Jahr: 2011
• Jahr: 11 November 2010
• Umfang: 16 S.
• Fussnoten: Gesehen am 04.05.2017
• Titel Quelle: Enthalten in: Plant, cell & environment
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1978
• Jahr Quelle: 2011
• Band/Heft Quelle: 34(2011), 2, Seite 192-207
• ISSN Quelle: 1365-3040
• DOI: doi:10.1111/j.1365-3040.2010.02235.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: broccoli
• Sach-SW: cancer prevention
• Sach-SW: cysteine (Cys)
• Sach-SW: glucosinolate (GSL)
• Sach-SW: glutathione (GSH)
• Sach-SW: MYB transcription factor
• Sach-SW: selenium (Se)
• Sach-SW: sulfate transporter (SULTR)
• K10plus-PPN: 1557941033

Abstract

Selenium (Se)-fortified broccoli (Brassica oleracea var. italica) has been proposed as a functional food for cancer prevention, based on its high glucosinolate (GSL) content and capacity for Se accumulation. However, as selenate and sulphate share the initial assimilation route, Se fertilization could interfere with sulphur metabolism and plant growth. Consequently, GSL accumulation could be compromised. To evaluate these potentially adverse effects of Se fertilization, we performed a comprehensive study on sand-grown young broccoli plants (weekly selenate applications of 0.8 µmol plant−1 via the root) and field-grown adult broccoli plants during head formation (single foliar selenate application: 25.3 or 253 µmol plant−1). The results show that under these conditions, Se application does not affect plant growth, contents of cysteine, glutathione, total GSL, glucoraphanin (major aliphatic GSL) or the expression of BoMYB28 (encoding a functionally confirmed master regulator for aliphatic GSL biosynthesis). Conversely, due to the changed expression of sulphate transporters (BoSULTR1;1, 1;2, 2;1, and 2;2), sulphate and total S contents increased in the shoot of young plants while decreasing in the root. We conclude that broccoli can be fertilized with Se without reduction in GSL content, even with Se accumulation exceeding the level recommended for human consumption.