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Verfasst von:Wang, Yi [VerfasserIn]   i
 Krebs, Melanie [VerfasserIn]   i
 Schumacher, Karin [VerfasserIn]   i
Titel:Cytosolic Ca2+ signals enhance the vacuolar ion conductivity of bulging arabidopsis root hair cells
Verf.angabe:Yi Wang, Julian Dindas, Florian Rienmüller, Melanie Krebs, Rainer Waadt, Karin Schumacher, Wei-Hua Wu, Rainer Hedrich, M. Rob G. Roelfsema
E-Jahr:2015
Jahr:2 November 2015
Umfang:10 S.
Fussnoten:Im Titel ist "2+" hochgestellt ; Gesehen am 05.05.2017
Titel Quelle:Enthalten in: Molecular plant
Ort Quelle:[Cambridge, MA] : Cell Press, 2008
Jahr Quelle:2015
Band/Heft Quelle:8(2015), 11, Seite 1665-1674
ISSN Quelle:1752-9867
Abstract:Plant cell expansion depends on the uptake of solutes across the plasma membrane and their storage within the vacuole. In contrast to the well-studied plasma membrane, little is known about the regulation of ion transport at the vacuolar membrane. We therefore established an experimental approach to study vacuolar ion transport in intact Arabidopsis root cells, with multi-barreled microelectrodes. The subcellular position of electrodes was detected by imaging current-injected fluorescent dyes. Comparison of measurements with electrodes in the cytosol and vacuole revealed an average vacuolar membrane potential of −31 mV. Voltage clamp recordings of single vacuoles resolved the activity of voltage-independent and slowly deactivating channels. In bulging root hairs that express the Ca2+ sensor R-GECO1, rapid elevation of the cytosolic Ca2+ concentration was observed, after impalement with microelectrodes, or injection of the Ca2+ chelator BAPTA. Elevation of the cytosolic Ca2+ level stimulated the activity of voltage-independent channels in the vacuolar membrane. Likewise, the vacuolar ion conductance was enhanced during a sudden increase of the cytosolic Ca2+ level in cells injected with fluorescent Ca2+ indicator FURA-2. These data thus show that cytosolic Ca2+ signals can rapidly activate vacuolar ion channels, which may prevent rupture of the vacuolar membrane, when facing mechanical forces.
DOI:doi:10.1016/j.molp.2015.07.009
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1016/j.molp.2015.07.009
 Volltext: http://www.sciencedirect.com/science/article/pii/S1674205215003032
 DOI: https://doi.org/10.1016/j.molp.2015.07.009
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Arabidopsis
 calcium signaling/transport
 electrophysiology
 fluorescence imaging
 ion channels
 vacuolar membrane
K10plus-PPN:1558265953
Verknüpfungen:→ Zeitschrift

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