Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms

• Verfasst von: Dettmer, Jan [VerfasserIn]
• Verfasst von: Liu, Tzu-Yin [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Titel: Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms
• Verf.angabe: Jan Dettmer, Tzu-Yin Liu, Karin Schumacher
• Jahr des Originals: 2009
• Umfang: 5 S.
• Fussnoten: Online published 28 November 2009 ; Gesehen am 10.05.2017
• Titel Quelle: Enthalten in: European journal of cell biology
• Jahr Quelle: 2010
• Band/Heft Quelle: 89(2010), 2-3, S. 152-156
• ISSN Quelle: 1618-1298
• DOI: doi:10.1016/j.ejcb.2009.11.008
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558393218

Abstract

Acidification of endomembrane compartments by the vacuolar H+-ATPase (V-ATPase) is an important mechanism to generate microenvironments suitable for various cellular functions. Differential assembly of subunit isoforms provides the potential to flexibly adapt the proton-pumping V-ATPase complex to changing physiological conditions and cell type-specific requirements. In Arabidopsis, the regulatory V-ATPase subunit E (VHA-E) is encoded by three genes with distinct expression patterns. We show here that VHA-E2, which belongs to a clade of pollen-specific VHA-E isoforms present in all higher plants, has a specialized but non-essential function during gametophyte development. Similarly, loss of the epidermis-specific isoform VHA-E3, which we show here to be transcriptionally regulated by the phytohormone jasmonic acid, does not cause obvious phenotypic changes. Furthermore, when expressed ubiquitously, VHA-E3, in contrast to VHA-E2, is able to complement loss of the essential subunit VHA-E1 indicating different degrees of functional specialization among the Arabidopsis VHA-E isoforms.