Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle

• Verfasst von: Viotti, Corrado [VerfasserIn]
• Verfasst von: Bubeck, Julia [VerfasserIn]
• Verfasst von: Krebs, Melanie [VerfasserIn]
• Verfasst von: Langhans, Markus [VerfasserIn]
• Verfasst von: Robinson, David G. [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Titel: Endocytic and secretory traffic in Arabidopsis merge in the trans-Golgi network/early endosome, an independent and highly dynamic organelle
• Verf.angabe: Corrado Viotti, Julia Bubeck, York-Dieter Stierhof, Melanie Krebs, Markus Langhans, Willy van den Berg, Walter van Dongen, Sandra Richter, Niko Geldner, Junpei Takano, Gerd Jürgens, Sacco C. de Vries, David G. Robinson, Karin Schumacher
• Umfang: 14 S.
• Fussnoten: Gesehen am 10.05.2017
• Titel Quelle: Enthalten in: The plant cell
• Jahr Quelle: 2010
• Band/Heft Quelle: 22(2010), 4, S. 1344-1357
• ISSN Quelle: 1532-298X
• DOI: doi:10.1105/tpc.109.072637
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 155840824X

Abstract

Plants constantly adjust their repertoire of plasma membrane proteins that mediates transduction of environmental and developmental signals as well as transport of ions, nutrients, and hormones. The importance of regulated secretory and endocytic trafficking is becoming increasingly clear; however, our knowledge of the compartments and molecular machinery involved is still fragmentary. We used immunogold electron microscopy and confocal laser scanning microscopy to trace the route of cargo molecules, including the BRASSINOSTEROID INSENSITIVE1 receptor and the REQUIRES HIGH BORON1 boron exporter, throughout the plant endomembrane system. Our results provide evidence that both endocytic and secretory cargo pass through the trans-Golgi network/early endosome (TGN/EE) and demonstrate that cargo in late endosomes/multivesicular bodies is destined for vacuolar degradation. Moreover, using spinning disc microscopy, we show that TGN/EEs move independently and are only transiently associated with an individual Golgi stack.