Methods for generating year-round access to amphioxus in the laboratory

• Verfasst von: Benito-Gutiérrez, Èlia [VerfasserIn]
• Verfasst von: Bryant, Diana [VerfasserIn]
• Verfasst von: Arendt, Detlev [VerfasserIn]
• Titel: Methods for generating year-round access to amphioxus in the laboratory
• Verf.angabe: Èlia Benito-Gutiérrez, Hermann Weber, Diana Virginia Bryant, Detlev Arendt
• Fussnoten: Gesehen am 12.05.2017
• Titel Quelle: Enthalten in: Public Library of Science: PLoS one
• Jahr Quelle: 2013
• Band/Heft Quelle: 8(2013,8) Artikel-Nummer e71599, 7 Seiten
• ISSN Quelle: 1932-6203
• DOI: doi:10.1371/journal.pone.0071599
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558546537

Abstract

Cephalochordates, commonly known as amphioxus, are key to understanding vertebrate origins. However, laboratory work suffers from limited access to adults and embryonic material. Here we report the design and experimental validation of an inland marine facility that allows establishing stable amphioxus colonies in the laboratory and obtaining embryos at any time of day and over almost the entire year, far exceeding natural conditions. This is achieved by mimicking the natural benthic environment, natural day- and moon- light, natural substrate and by providing a strictly controlled and seasonally fluctuating temperature regimen. Moreover, supplemented algae diets allow animals to refill their gonads in consecutive years. Spontaneous spawning, a major problem in previous setups, no longer occurs in our facility; instead, all breeding is induced and fertilization occurs fully in vitro. Our system makes amphioxus a standard laboratory animal model.