| Online-Ressource |
Verfasst von: | Hagen, Ilja [VerfasserIn]  |
| Großmann, Guido [VerfasserIn]  |
| Hauser, Nicole [VerfasserIn]  |
| Hoheisel, Jörg D. [VerfasserIn]  |
Titel: | Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins |
Verf.angabe: | Ilja Hagen, Margit Ecker, Arnaud Lagorce, Jean M. Francois, Sergej Sestak, Reinhard Rachel, Guido Grossmann, Nicole C. Hauser, Jörg D. Hoheisel, Widmar Tanner and Sabine Strahl |
E-Jahr: | 2004 |
Jahr: | 30 January, 2004 |
Umfang: | 13 S. |
Fussnoten: | Gesehen am 12.05.2017 |
Titel Quelle: | Enthalten in: Molecular microbiology |
Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1987 |
Jahr Quelle: | 2004 |
Band/Heft Quelle: | 52(2004), 5, Seite 1413-1425 |
ISSN Quelle: | 1365-2958 |
Abstract: | The covalently linked cell wall protein Ccw12p of Saccharomyces cerevisiae is a GPI-anchored protein (V. Mrsa et al., 1999, J Bacteriol 181: 3076-3086). Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of >300 kDa. A membrane-bound form with an apparent molecular mass of 58 kDa is highly O- and N-glycosylated and contains the GPI anchor. With a half-life of 2 min, the membrane form is transformed to the >300 kDa form. The deletion mutant ccw12Δ grows slower than the wild type, is highly sensitive to Calcofluor white and contains 2.5 times more chitin. Further, compared with wild-type yeast, significantly more proteins are released from intact cells when treated with dithiothreitol. Interestingly, these defects become less pronounced when further GPI-anchored cell wall proteins are deleted. Mutant ΔGPI (simultaneous deletion of CCW12, CCW13/DAN1, CCW14, TIP1 and CWP1) is similar in many respects to wild-type yeast. To find out how the cell wall is stabilized in mutant ΔGPI, a genome-wide transcription analysis was performed. Of 159 significantly regulated genes, 14 encode either known or suspected cell wall-associated proteins. Analysis of genes affected in transcription revealed that SED1 and SRL1 in particular are required to reconstruct cell wall stability in the absence of multiple GPI-anchored mannoproteins. |
DOI: | doi:10.1111/j.1365-2958.2004.04064.x |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: http://dx.doi.org/10.1111/j.1365-2958.2004.04064.x |
| kostenfrei: Volltext: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04064.x/abstract |
| DOI: https://doi.org/10.1111/j.1365-2958.2004.04064.x |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1558570365 |
Verknüpfungen: | → Zeitschrift |
Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins / Hagen, Ilja [VerfasserIn]; 30 January, 2004 (Online-Ressource)