Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins

• Verfasst von: Hagen, Ilja [VerfasserIn]
• Verfasst von: Großmann, Guido [VerfasserIn]
• Verfasst von: Hauser, Nicole [VerfasserIn]
• Verfasst von: Hoheisel, Jörg D. [VerfasserIn]
• Titel: Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins
• Verf.angabe: Ilja Hagen, Margit Ecker, Arnaud Lagorce, Jean M. Francois, Sergej Sestak, Reinhard Rachel, Guido Grossmann, Nicole C. Hauser, Jörg D. Hoheisel, Widmar Tanner and Sabine Strahl
• E-Jahr: 2004
• Jahr: 30 January, 2004
• Umfang: 13 S.
• Fussnoten: Gesehen am 12.05.2017
• Titel Quelle: Enthalten in: Molecular microbiology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1987
• Jahr Quelle: 2004
• Band/Heft Quelle: 52(2004), 5, Seite 1413-1425
• ISSN Quelle: 1365-2958
• DOI: doi:10.1111/j.1365-2958.2004.04064.x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558570365

Abstract

The covalently linked cell wall protein Ccw12p of Saccharomyces cerevisiae is a GPI-anchored protein (V. Mrsa et al., 1999, J Bacteriol 181: 3076-3086). Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of >300 kDa. A membrane-bound form with an apparent molecular mass of 58 kDa is highly O- and N-glycosylated and contains the GPI anchor. With a half-life of 2 min, the membrane form is transformed to the >300 kDa form. The deletion mutant ccw12Δ grows slower than the wild type, is highly sensitive to Calcofluor white and contains 2.5 times more chitin. Further, compared with wild-type yeast, significantly more proteins are released from intact cells when treated with dithiothreitol. Interestingly, these defects become less pronounced when further GPI-anchored cell wall proteins are deleted. Mutant ΔGPI (simultaneous deletion of CCW12, CCW13/DAN1, CCW14, TIP1 and CWP1) is similar in many respects to wild-type yeast. To find out how the cell wall is stabilized in mutant ΔGPI, a genome-wide transcription analysis was performed. Of 159 significantly regulated genes, 14 encode either known or suspected cell wall-associated proteins. Analysis of genes affected in transcription revealed that SED1 and SRL1 in particular are required to reconstruct cell wall stability in the absence of multiple GPI-anchored mannoproteins.