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Verfasst von:Bogs, Jochen [VerfasserIn]   i
 Bruchmüller, Iris [VerfasserIn]   i
 Erbar, Claudia [VerfasserIn]   i
 Geider, Klaus [VerfasserIn]   i
Titel:Colonization of host plants by the fire blight pathogen Erwinia amylovora marked with genes for nioluminescence and fluorescence
Verf.angabe:Jochen Bogs, Iris Bruchmüller, Claudia Erbar, and Klaus Geider
E-Jahr:1998
Jahr:17 January 1998
Umfang:6 S.
Fussnoten:Gesehen am 15.05.2017
Titel Quelle:Enthalten in: Phytopathology
Ort Quelle:Saint Paul, Minn. : APS, 1970
Jahr Quelle:1998
Band/Heft Quelle:88(1998), 5, Seite 416-421
ISSN Quelle:1943-7684
Abstract:To follow the movement of Erwinia amylovora in plant tissue without dissection, this bacterium was marked with either the lux operon from Vibrio fischeri or the gfp gene from the jellyfish Aequorea victoria, both carried on multicopy plasmids and expressed under the control of the lac promoter from Escherichia coli. Movement of the pathogen was visualized in leaves, stems, and roots of apple seedlings, and migration of E. amylovora was traced from inoculation sites in the stem to as far as the roots. Green fluorescent E. amylovora cells were observed in the xylem and later appeared to break out of the vessels into the intercellular spaces of the adjacent parenchyma. Inoculation in the intercostal region of leaves caused a zone of slow necrosis that finally resulted in bacterial invasion of the xylem vessels. Labeled bacteria could also be seen in association with the anchor sites of leaf hairs. Distortion of the epidermis adjacent to leaf hairs created openings that were observed by scanning electron microscopy. As the intercostal region, the bases of leaf hairs provided E. amylovora access to intact xylem vessels, which allowed further distribution of the pathogen in the host plant.
DOI:doi:10.1094/PHYTO.1998.88.5.416
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: http://dx.doi.org/10.1094/PHYTO.1998.88.5.416
 Volltext: http://apsjournals.apsnet.org/doi/10.1094/PHYTO.1998.88.5.416
 DOI: https://doi.org/10.1094/PHYTO.1998.88.5.416
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1558598987
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