Modulation of chloride homeostasis by inflammatory mediators in dorsal root ganglion neurons

• Verfasst von: Lehmeyer-Funk, Katharina [VerfasserIn]
• Verfasst von: Woitecki, Anne [VerfasserIn]
• Verfasst von: Franjic-Würtz, Christina [VerfasserIn]
• Verfasst von: Möhrlen, Frank [VerfasserIn]
• Verfasst von: Frings, Stephan [VerfasserIn]
• Titel: Modulation of chloride homeostasis by inflammatory mediators in dorsal root ganglion neurons
• Verf.angabe: Katharina Funk, Anne Woitecki, Christina Franjic-Würtz, Thomas Gensch, Frank Möhrlen and Stephan Frings
• Umfang: 12 S.
• Fussnoten: Gesehen am 15.05.2017
• Titel Quelle: Enthalten in: Molecular pain
• Jahr Quelle: 2008
• Band/Heft Quelle: 4(2008), S. 1-12
• ISSN Quelle: 1744-8069
• DOI: doi:10.1186/1744-8069-4-32
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558616454

Abstract

Background:Chloride currents in peripheral nociceptive neurons have been implicated in the generation of afferent nociceptive signals, as Cl− accumulation in sensory endings establishes the driving force for depolarizing, and even excitatory, Cl− currents. The intracellular Cl− concentration can, however, vary considerably between individual DRG neurons. This raises the question, whether the contribution of Cl− currents to signal generation differs between individual afferent neurons, and whether the specific Cl− levels in these neurons are subject to modulation. Based on the hypothesis that modulation of the peripheral Cl− homeostasis is involved in the generation of inflammatory hyperalgesia, we examined the effects of inflammatory mediators on intracellular Cl− concentrations and on the expression levels of Cl− transporters in rat DRG neurons.Results:We developed an in vitro assay for testing how inflammatory mediators influence Cl− concentration and the expression of Cl− transporters. Intact DRGs were...