Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Lam, Sheung Kwan [VerfasserIn]  |
| Hillmer, Stefan [VerfasserIn]  |
| Robinson, David G. [VerfasserIn]  |
Titel: | Rice SCAMP1 defines clathrin-coated, trans-Golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells |
Verf.angabe: | Sheung Kwan Lam, Ching Lung Siu, Stefan Hillmer, Seonghoe Jang, Gynheung An, David G. Robinson and Liwen Jiang |
Umfang: | 24 S. |
Fussnoten: | Gesehen am 17.05.2017 |
Titel Quelle: | Enthalten in: The plant cell |
Jahr Quelle: | 2007 |
Band/Heft Quelle: | 19(2007), 1, S. 296-319 |
ISSN Quelle: | 1532-298X |
Abstract: | We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to the lytic vacuole in tobacco (Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are post-Golgi, integral membrane proteins mediating endocytosis in animal cells. To define the endocytic pathway in plants, we cloned the rice (Oryza sativa) homolog of animal SCAMP1 and generated transgenic tobacco BY-2 cells expressing yellow fluorescent protein (YFP)-SCAMP1 or SCAMP1-YFP fusions. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that YFP-SCAMP1 fusions and native SCAMP1 localize to the plasma membrane and mobile structures in the cytoplasm of transgenic BY-2 cells. Drug treatments and confocal immunofluorescence studies demonstrated that the punctate cytosolic organelles labeled by YFP-SCAMP1 or SCAMP1 were distinct from the Golgi apparatus and PVCs. SCAMP1-labeled organelles may represent an early endosome because the internalized endocytic markers FM4-64 and AM4-64 reached these organelles before PVCs. In addition, wortmannin caused the redistribution of SCAMP1 from the early endosomes to PVCs, probably as a result of fusions between the two compartments. Immunogold electron microscopy with high-pressure frozen/freeze-substituted samples identified the SCAMP1-positive organelles as tubular-vesicular structures at the trans-Golgi with clathrin coats. These early endosomal compartments resemble the previously described partially coated reticulum and trans-Golgi network in plant cells. |
DOI: | doi:10.1105/tpc.106.045708 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Verlag: http://dx.doi.org/10.1105/tpc.106.045708 |
| Kostenfrei: Verlag: http://www.plantcell.org/content/19/1/296 |
| DOI: https://doi.org/10.1105/tpc.106.045708 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1558699848 |
Verknüpfungen: | → Zeitschrift |
Rice SCAMP1 defines clathrin-coated, trans-Golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells / Lam, Sheung Kwan [VerfasserIn] (Online-Ressource)
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