Jasmonate signaling in the field, part II

• Verfasst von: Gaquerel, Emmanuel [VerfasserIn]
• Verfasst von: Stitz, Michael [VerfasserIn]
• Verfasst von: Kallenbach, Mario [VerfasserIn]
• Verfasst von: Baldwin, Ian T. [VerfasserIn]
• Titel: Jasmonate signaling in the field, part II
• Titelzusatz: insect-guided characterization of genetic variations in Jasmonate-dependent defenses of transgenic and natural Nicotiana attenuata populations
• Verf.angabe: Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, IanT. Baldwin
• E-Jahr: 2013
• Jahr: 03 April 2013
• Umfang: 13 S.
• Teil: year:2013
• Teil: pages:97-109
• Teil: extent:13
• Fussnoten: Gesehen am 18.05.2017
• Titel Quelle: Enthalten in: Goossens, AlainJasmonate Signaling
• Ort Quelle: Totowa, NJ : Humana Press, 2013
• Jahr Quelle: 2013
• Band/Heft Quelle: (2013), Seite 97-109
• ISBN Quelle: 978-1-62703-414-2
• DOI: doi:10.1007/978-1-62703-414-2_8
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Field work
• Sach-SW: Induced defenses
• Sach-SW: Jasmonate
• Sach-SW: Plant-insect interaction
• Sach-SW: Proteinase inhibitors
• Sach-SW: Secondary metabolites
• K10plus-PPN: 1558766634

Abstract

The introduction of genetically modified plants into natural habitats represents a valuable means to determine organismic level functions of a gene and its effects on a plant’s interaction with other organisms. Nicotiana attenuata, a wild tobacco species native of the southwestern USA that grows in the immediate postfire environment, is one of the important host plants for herbivore populations recolonizing recently burned habitats in the Great Basin Desert. Here, we provide detailed guidelines for the analysis, under field conditions, of jasmonate-dependent defense and its impact on the plant’s native herbivore community. The procedures are based on the field release of transgenic lines silenced for jasmonate biogenesis, metabolism, or perception to conduct association studies between defense trait expression (secondary metabolite and trypsin proteinase inhibitor accumulation) and insect infestations. Additionally, because some insects have evolved mechanisms to “eavesdrop” on jasmonate signaling when selecting their host plants, we describe how leafhoppers of the species Empoasca, which selectively colonize jasmonate-deficient plants, can be used as “bloodhounds” for identifying natural variations in jasmonate signaling among natural N. attenuata populations.