Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels

• Verfasst von: Yoshikawa, Hiroshi [VerfasserIn]
• Verfasst von: Rossetti, Fernanda [VerfasserIn]
• Verfasst von: Kaufmann, Stefan [VerfasserIn]
• Verfasst von: Kaindl, Thomas [VerfasserIn]
• Verfasst von: Engel, Ulrike [VerfasserIn]
• Verfasst von: Tanaka, Motomu [VerfasserIn]
• Titel: Quantitative evaluation of mechanosensing of cells on dynamically tunable hydrogels
• Verf.angabe: Hiroshi Y. Yoshikawa, Fernanda F. Rossetti, Stefan Kaufmann, Thomas Kaindl, Jeppe Madsen, Ulrike Engel, Andrew L. Lewis, Steven P. Armes, Motomu Tanaka
• Umfang: 8 S.
• Fussnoten: Gesehen am 22.05.2017
• Titel Quelle: Enthalten in: American Chemical Society: Journal of the American Chemical Society
• Jahr Quelle: 2011
• Band/Heft Quelle: 133(2011), 5, S. 1367-1374
• ISSN Quelle: 1520-5126
• DOI: doi:10.1021/ja1060615
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558841474

Abstract

Thin hydrogel films based on an ABA triblock copolymer gelator [where A is pH-sensitive poly(2-(diisopropylamino)ethyl methacrylate) (PDPA) and B is biocompatible poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC)] were used as a stimulus-responsive substrate that allows fine adjustment of the mechanical environment experienced by mouse myoblast cells. The hydrogel film elasticity could be reversibly modulated by a factor of 40 via careful pH adjustment without adversely affecting cell viability. Myoblast cells exhibited pronounced stress fiber formation and flattening on increasing the hydrogel elasticity. As a new tool to evaluate the strength of cell adhesion, we combined a picosecond laser with an inverted microscope and utilized the strong shock wave created by the laser pulse to determine the critical pressure required for cell detachment. Furthermore, we demonstrate that an abrupt jump in the hydrogel elasticity can be utilized to monitor how cells adapt their morphology to changes in their mechanical environment.