CSC-1

• Verfasst von: Romano, Alper [VerfasserIn]
• Verfasst von: Guse, Annika [VerfasserIn]
• Titel: CSC-1
• Titelzusatz: a subunit of the Aurora B kinase complex that binds to the Survivin-like protein BIR-1 and the Incenp-like protein ICP-1
• Verf.angabe: Alper Romano, Annika Guse, Ivica Krascenicova, Heinke Schnabel, Ralf Schnabel, and Michael Glotzer
• Umfang: 8 S.
• Fussnoten: Gesehen am 22.05.2017
• Titel Quelle: Enthalten in: The journal of cell biology
• Jahr Quelle: 2003
• Band/Heft Quelle: 161(2003), 2, S. 229-236
• ISSN Quelle: 1540-8140
• DOI: doi:10.1083/jcb.200207117
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558882707

Abstract

The Aurora B kinase complex is a critical regulator of chromosome segregation and cytokinesis. In Caenorhabditis elegans, AIR-2 (Aurora B) function requires ICP-1 (Incenp) and BIR-1 (Survivin). In various systems, Aurora B binds to orthologues of these proteins. Through genetic analysis, we have identified a new subunit of the Aurora B kinase complex, CSC-1. C. elegans embryos depleted of CSC-1, AIR-2, ICP-1, or BIR-1 have identical phenotypes. CSC-1, BIR-1, and ICP-1 are interdependent for their localization, and all are required for AIR-2 localization. In vitro, CSC-1 binds directly to BIR-1. The CSC-1/BIR-1 complex, but not the individual subunits, associates with ICP-1. CSC-1 associates with ICP-1, BIR-1, and AIR-2 in vivo. ICP-1 dramatically stimulates AIR-2 kinase activity. This activity is not stimulated by CSC-1/BIR-1, suggesting that these two subunits function as targeting subunits for AIR-2 kinase.