Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of SDF2-like protein from Arabidopsis thaliana

• Verfasst von: Radzimanowski, Jens [VerfasserIn]
• Verfasst von: Ravaud, Stéphanie [VerfasserIn]
• Verfasst von: Schott, Andrea [VerfasserIn]
• Verfasst von: Strahl, Sabine [VerfasserIn]
• Verfasst von: Sinning, Irmgard [VerfasserIn]
• Titel: Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of SDF2-like protein from Arabidopsis thaliana
• Verf.angabe: Jens Radzimanowski, Stephanie Ravaud, Andrea Schott, Sabine Strahl and Irmgard Sinning
• E-Jahr: 2010
• Jahr: January 2010
• Umfang: 3 S.
• Fussnoten: Gesehen am 23.05.2017
• Titel Quelle: Enthalten in: Acta crystallographica / F
• Ort Quelle: Oxford [u.a.] : Blackwell, 2005
• Jahr Quelle: 2010
• Band/Heft Quelle: 66(2010), 1, Seite 12-14
• ISSN Quelle: 2053-230X
• ISSN Quelle: 1744-3091
• DOI: doi:10.1107/S1744309109042018
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558925945

Abstract

The stromal-cell-derived factor 2-like protein of Arabidopsis thaliana (AtSDL) has been shown to be highly up-regulated in response to unfolded protein response (UPR) inducing reagents, suggesting that it plays a crucial role in the plant UPR pathway. AtSDL has been cloned, overexpressed, purified and crystallized using the vapour-diffusion method. Two crystal forms have been obtained under very similar conditions. The needle-shaped crystals did not diffract X-rays, while the other form diffracted to 1.95 Å resolution using a synchrotron-radiation source and belonged to the hexagonal space group P61, with unit-cell parameters a = b = 96.1, c = 69.3 Å.