The oriented emergence of axons from retinal ganglion cells is directed by laminin contact in vivo

• Verfasst von: Randlett, Owen [VerfasserIn]
• Verfasst von: Poggi, Lucia [VerfasserIn]
• Verfasst von: Zolessi, Flavio R. [VerfasserIn]
• Verfasst von: Harris, William A. [VerfasserIn]
• Titel: The oriented emergence of axons from retinal ganglion cells is directed by laminin contact in vivo
• Verf.angabe: Owen Randlett, Lucia Poggi, Flavio R. Zolessi, and William A. Harris
• E-Jahr: 2011
• Jahr: [April 28, 2011]
• Umfang: 15 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 24.05.2017
• Titel Quelle: Enthalten in: Neuron
• Ort Quelle: [Cambridge, Mass.] : Cell Press, 1988
• Jahr Quelle: 2011
• Band/Heft Quelle: 70(2011), 2, Seite 266-280
• ISSN Quelle: 1097-4199
• DOI: doi:10.1016/j.neuron.2011.03.013
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1558991778

Abstract

Summary: How the site of axon emergence is specified during neural development is not understood. Previous studies disagree on the relative importance of intrinsic and extrinsic mechanisms. The axons of retinal ganglion cells (RGCs) emerge basally in vivo, yet because RGCs develop from polarized neuroepithelial cells within a polarized environment, disentangling intrinsic and extrinsic influences is a challenge. We use time-lapse imaging to demonstrate that Laminin acting directly on RGCs is necessary and sufficient to orient axon emergence in vivo. Laminin contact with the basal processes of newborn RGCs prevents the cells from entering a stochastic Stage 2 phase, directs the rapid accumulation of the early axonal marker Kif5c560-YFP, and leads to the formation of axonal growth cones. These results suggest that contact-mediated cues may be critical for the site of axon emergence and account for the differences in cellular behavior observed in vitro and in vivo. Video Abstract