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Verfasst von:Keinath, Nana [VerfasserIn]   i
 Waadt, Rainer [VerfasserIn]   i
 Brugman, Rik [VerfasserIn]   i
 Großmann, Guido [VerfasserIn]   i
 Schumacher, Karin [VerfasserIn]   i
 Krebs, Melanie [VerfasserIn]   i
Titel:Live cell imaging with R-GECO1 sheds light on flg22- and Chitin-induced transient [Ca2+]cyt patterns in Arabidopsis
Verf.angabe:Nana F. Keinath, Rainer Waadt, Rik Brugman, Julian I. Schroeder, Guido Grossmann, Karin Schumacher, Melanie Krebs
E-Jahr:2015
Jahr:19 May 2015
Umfang:13 S.
Fussnoten:Gesehen am 07.06.2017
Titel Quelle:Enthalten in: Molecular plant
Ort Quelle:[Cambridge, MA] : Cell Press, 2008
Jahr Quelle:2015
Band/Heft Quelle:8(2015), 8, Seite 1188-1200
ISSN Quelle:1752-9867
Abstract:Intracellular Ca2+ transients are an integral part of the signaling cascade during pathogen-associated molecular pattern (PAMP)-triggered immunity in plants. Yet, our knowledge about the spatial distribution of PAMP-induced Ca2+ signals is limited. Investigation of cell- and tissue-specific properties of Ca2+-dependent signaling processes requires versatile Ca2+ reporters that are able to extract spatial information from cellular and subcellular structures, as well as from whole tissues over time periods from seconds to hours. Fluorescence-based reporters cover both a broad spatial and temporal range, which makes them ideally suited to study Ca2+ signaling in living cells. In this study, we compared two fluorescence-based Ca2+ sensors: the Förster resonance energy transfer (FRET)-based reporter yellow cameleon NES-YC3.6 and the intensity-based sensor R-GECO1. We demonstrate that R-GECO1 exhibits a significantly increased signal change compared with ratiometric NES-YC3.6 in response to several stimuli. Due to its superior sensitivity, R-GECO1 is able to report flg22- and chitin-induced Ca2+ signals on a cellular scale, which allowed identification of defined [Ca2+]cyt oscillations in epidermal and guard cells in response to the fungal elicitor chitin. Moreover, we discovered that flg22- and chitin-induced Ca2+ signals in the root initiate from the elongation zone.
DOI:doi:10.1016/j.molp.2015.05.006
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1016/j.molp.2015.05.006
 Volltext: http://www.sciencedirect.com/science/article/pii/S1674205215002397
 DOI: https://doi.org/10.1016/j.molp.2015.05.006
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1559503106
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