Effects of low seawater pH on the marine polychaete Platynereis dumerilii

• Verfasst von: Wäge, Janine [VerfasserIn]
• Verfasst von: Larsson, Tomas [VerfasserIn]
• Verfasst von: Simakov, Oleg [VerfasserIn]
• Verfasst von: Arendt, Detlev [VerfasserIn]
• Titel: Effects of low seawater pH on the marine polychaete Platynereis dumerilii
• Verf.angabe: Janine Wäge, Jörg D. Hardege, Tomas A. Larsson, Oleg Simakov, Emma C. Chapman, Detlev Arendt, Jeanette M. Rotchell
• Umfang: 7 S.
• Fussnoten: Gesehen am 09.06.2017
• Titel Quelle: Enthalten in: Marine pollution bulletin
• Jahr Quelle: 2015
• Band/Heft Quelle: 95(2015), 1, S. 166-172
• ISSN Quelle: 1879-3363
• DOI: doi:10.1016/j.marpolbul.2015.04.027
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1559620617

Abstract

An important priority for any organism is to maintain internal cellular homeostasis including acid-base balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1h and up-regulated after 7days under low pH treatment (pH 7.8), indicating changes in acid-base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1h was also observed, suggesting a shift in the CO2 and HCO3− balance. No change in CaM expression was detected after 7days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism.