Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture

• Verfasst von: Zhai, Zongzhao [VerfasserIn]
• Verfasst von: Yang, Xingke [VerfasserIn]
• Verfasst von: Lohmann, Ingrid [VerfasserIn]
• Titel: Functional dissection of the Hox protein Abdominal-B in Drosophila cell culture
• Verf.angabe: Zongzhao Zhai, Xingke Yang, Ingrid Lohmann
• Umfang: 6 S.
• Fussnoten: Gesehen am 13.06.2017
• Titel Quelle: Enthalten in: Biochemical and biophysical research communications
• Jahr Quelle: 2011
• Band/Heft Quelle: 414(2011), 4, S. 761-766
• ISSN Quelle: 1090-2104
• DOI: doi:10.1016/j.bbrc.2011.09.154
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1559701056

Abstract

Hox transcription factors regulate the morphogenesis along the anterior-posterior (A/P) body axis through the interaction with small cis-regulatory modules (CRMs) of their target gene, however so far very few Hox CRMs are known and have been analyzed in detail. In this study we have identified a new Hox CRM, ct340, which guides the expression of the cell type specification gene cut (ct) in the posterior spiracle under the direct control of the Hox protein Abdominal-B (Abd-B). Using the ct340 enhancer activity as readout, an efficient cloning system to generate VP16 activation domain fusion protein was developed to unambiguously test protein-DNA interaction in Drosophila cell culture. By functionally dissecting the Abd-B protein, new features of Abd-B dependent target gene regulation were detected. Due to its easy adaptability, this system can be generally used to map functional domains within sequence-specific transcriptional factors in Drosophila cell culture, and thus provide preliminary knowledge of the protein functional domain structure for further in vivo analysis.