Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system

• Verfasst von: Boy, Aurelia Lara [VerfasserIn]
• Verfasst von: Zhai, Zongzhao [VerfasserIn]
• Verfasst von: Kussler-Schneider, Yvonne [VerfasserIn]
• Verfasst von: Kaspar, Petra [VerfasserIn]
• Verfasst von: Lohmann, Ingrid [VerfasserIn]
• Titel: Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system
• Verf.angabe: Aurelia L. Boy, Zongzhao Zhai, Anette Habring-Müller, Yvonne Kussler-Schneider, Petra Kaspar, Ingrid Lohmann
• Umfang: 5 S.
• Fussnoten: Gesehen am 13.06.2017
• Titel Quelle: Enthalten in: Genesis
• Jahr Quelle: 2010
• Band/Heft Quelle: 48(2010), 7, S. 452-456
• ISSN Quelle: 1526-968X
• DOI: doi:10.1002/dvg.20637
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1559703938

Abstract

The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins. genesis 48:452-456, 2010. © 2010 Wiley-Liss, Inc.