The tetraspanins CD151 and Tspan8 are essential exosome components for the crosstalk between cancer initiating cells and their surrounding

• Verfasst von: Yue, Shijing [VerfasserIn]
• Verfasst von: Mu, Wei [VerfasserIn]
• Verfasst von: Erb, Ulrike [VerfasserIn]
• Verfasst von: Zöller, Margot [VerfasserIn]
• Titel: The tetraspanins CD151 and Tspan8 are essential exosome components for the crosstalk between cancer initiating cells and their surrounding
• Verf.angabe: Shijing Yue, Wei Mu, Ulrike Erb and Margot Zöller
• Jahr: 2015
• Umfang: 19 S.
• Teil: volume:6
• Teil: year:2015
• Teil: number:4
• Teil: pages:2366-2384
• Teil: extent:19
• Fussnoten: Published online 2014 Dec 10 ; Gesehen am 14.07.2017
• Titel Quelle: Enthalten in: OncoTarget
• Ort Quelle: [S.l.] : Impact Journals LLC, 2010
• Jahr Quelle: 2015
• Band/Heft Quelle: 6(2015), 4, Seite 2366-2384
• ISSN Quelle: 1949-2553
• DOI: doi:10.18632/oncotarget.2958
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1560840056

Abstract

Tspan8 and CD151 are metastasis-promoting tetraspanins and a knockdown (kd) of Tspan8 or CD151 and most pronounced of both tetraspanins affects the metastatic potential of the rat pancreatic adenocarcinoma line ASML. Approaching to elaborate the underlying mechanism, we compared ASMLwt, -CD151kd and/or Tspan8kd clones. We focused on tumor exosomes, as exosomes play a major role in tumor progression and tetraspanins are suggested to be engaged in exosome targeting., ASML-CD151/Tspan8kd cells poorly metastasize, but regain metastatic capacity, when rats are pretreated with ASMLwt, but not ASML-CD151kd and/or -Tspan8kd exosomes. Both exosomal CD151 and Tspan8 contribute to host matrix remodelling due to exosomal tetraspanin-integrin and tetraspanin-protease associations. ASMLwt exosomes also support stroma cell activation with upregulation of cytokines, cytokine receptors and proteases and promote inflammatory cytokine expression in hematopoietic cells. Finally, CD151-/Tspan8-competent exosomes support EMT gene expression in poorly-metastatic ASML-CD151/Tspan8kd cells. These effects are not seen or are weakened using ASML-CD151kd or -Tspan8kd exosomes, which is at least partly due to reduced binding/uptake of CD151- and/or Tspan8-deficient exosomes., Thus, CD151- and Tspan8-competent tumor exosomes support matrix degradation, reprogram stroma and hematopoietic cells and drive non-metastatic ASML-CD151/Tspan8kd cells towards a motile phenotype.