Status: Bibliographieeintrag
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| Verfasst von: | Dettmer, Susan [VerfasserIn]  |
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| | Theile, Dirk [VerfasserIn] |
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| | Seckinger, Anja [VerfasserIn] |
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| | Burhenne, Jürgen [VerfasserIn] |
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| | Weiß, Johanna [VerfasserIn] |
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| Titel: | Fetal calf sera can distort cell-based luminescent proteasome assays through heat-resistant chymotrypsin-like activity |
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| Verf.angabe: | Susan Dettmer, Dirk Theile, Anja Seckinger, Jürgen Burhenne, Johanna Weiss |
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| Jahr: | 2015 |
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| Jahr des Originals: | 2014 |
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| Umfang: | 3 S. |
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| Fussnoten: | Published online: 4 November 2014 ; Gesehen am 17.07.2017 |
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| Titel Quelle: | Enthalten in: Analytical biochemistry |
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| Ort Quelle: | San Diego, Calif. : Elsevier, 1960 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 471(2015), Seite 23-25 |
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| ISSN Quelle: | 1096-0309 |
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| Abstract: | Luminescence-based proteasome activity assays use specific substrates that are supposed to be cleaved by cellular proteasome activity leading to luciferase substrates. Usually, control wells containing cell culture medium supplemented with antibiotics and fetal calf serum are used as background. Using the Proteasome-Glo chymotrypsin-like cell-based assay from Promega, we show here that fetal calf sera from different manufacturers contain heat-resistant, bortezomib-inhibitable, chymotrypsin-like activities that can interfere with proteasome activity assays. These data strongly recommend the use of pure phosphate-buffered saline (PBS) or serum-free medium during proteasome activity assays to diminish background luminescence and, thus, to obtain reliable results. |
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| DOI: | doi:10.1016/j.ab.2014.10.019 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.ab.2014.10.019 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0003269714005090 |
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| | DOI: https://doi.org/10.1016/j.ab.2014.10.019 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Bortezomib |
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| | Carfilzomib |
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| | Fetal calf serum |
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| | Heat resistance |
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| | Proteasome activity |
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| | Proteasome-Glo chymotrypsin-like cell-based assays |
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| K10plus-PPN: | 1560914068 |
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| Verknüpfungen: | → Zeitschrift |
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Fetal calf sera can distort cell-based luminescent proteasome assays through heat-resistant chymotrypsin-like activity / Dettmer, Susan [VerfasserIn]; 2015 (Online-Ressource)
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