Clinical proteomics

• Verfasst von: Betzen, Christian [VerfasserIn]
• Verfasst von: Hoheisel, Jörg D. [VerfasserIn]
• Titel: Clinical proteomics
• Titelzusatz: Promises, challenges and limitations of affinity arrays
• Verf.angabe: Christian Betzen, Mohamed Saiel Saeed Alhamdani, Smiths Lueong, Christoph Schröder, Axel Stang and Jörg D. Hoheisel
• Jahr: 2015
• Umfang: 6 S.
• Teil: volume:9
• Teil: year:2015
• Teil: number:3-4
• Teil: pages:342-347
• Teil: extent:6
• Fussnoten: First published: 2 March 2015 ; Gesehen am 21.07.2017
• Titel Quelle: Enthalten in: Proteomics / Clinical applications
• Ort Quelle: Weinheim : Wiley VCH, 2007
• Jahr Quelle: 2015
• Band/Heft Quelle: 9(2015), 3-4, Seite 342-347
• ISSN Quelle: 1862-8354
• DOI: doi:10.1002/prca.201400156
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Affinity profiling
• Sach-SW: Antibodies
• Sach-SW: Immunoassay
• Sach-SW: Interaction
• Sach-SW: Protein microarrays
• K10plus-PPN: 1561083860

Abstract

After the establishment of DNA/RNA sequencing as a means of clinical diagnosis, the analysis of the proteome is next in line. As a matter of fact, proteome-based diagnostics is bound to be even more informative, since proteins are directly involved in the actual cellular processes that are responsible for disease. However, the structural variation and the biochemical differences between proteins, the much wider range in concentration and their spatial distribution as well as the fact that protein activity frequently relies on interaction increase the methodological complexity enormously, particularly if an accuracy and robustness is required that is sufficient for clinical utility. Here, we discuss the contribution that protein microarray formats could play towards proteome-based diagnostics.