Single-cell analysis uncovers clonal acinar cell heterogeneity in the adult pancreas

• Verfasst von: Wollny, Damian [VerfasserIn]
• Verfasst von: Ziebell, Frederik [VerfasserIn]
• Verfasst von: Marciniak-Czochra, Anna [VerfasserIn]
• Titel: Single-cell analysis uncovers clonal acinar cell heterogeneity in the adult pancreas
• Verf.angabe: Damian Wollny, Sheng Zhao, Isabelle Everlien, Xiaokang Lun, Jan Brunken, Daniel Brüne, Frederik Ziebell, Inna Tabansky, Wilko Weichert, Anna Marciniak-Czochra, Ana Martin-Villalba
• Umfang: 13 S.
• Fussnoten: Gesehen am 27.07.2017
• Titel Quelle: Enthalten in: Developmental cell
• Jahr Quelle: 2016
• Band/Heft Quelle: 39(2016), 3, S. 289-301
• ISSN Quelle: 1878-1551
• DOI: doi:10.1016/j.devcel.2016.10.002
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1561252700

Abstract

Acinar cells make up the majority of all cells in the pancreas, yet the source of new acinar cells during homeostasis remains unknown. Using multicolor lineage-tracing and organoid-formation assays, we identified the presence of a progenitor-like acinar cell subpopulation. These cells have long-term self-renewal capacity, albeit in a unipotent fashion. We further demonstrate that binuclear acinar cells are terminally differentiated acinar cells. Transcriptome analysis of single acinar cells revealed the existence of a minor population of cells expressing progenitor markers. Interestingly, a gain of the identified markers accompanied by a transient gain of proliferation was observed following chemically induced pancreatitis. Altogether, our study identifies a functionally and molecularly distinct acinar subpopulation and thus transforms our understanding of the acinar cell compartment as a pool of equipotent secretory cells.