Cucurbitacins

• Verfasst von: Wang, Xiaojuan [VerfasserIn]
• Verfasst von: Tanaka, Mine [VerfasserIn]
• Verfasst von: Peixoto, Herbenya [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Cucurbitacins
• Titelzusatz: elucidation of their interactions with the cytoskeleton
• Verf.angabe: Xiaojuan Wang, Mine Tanaka, Herbenya Silva Peixoto and Michael Wink
• E-Jahr: 2017
• Jahr: 30 May 2017
• Umfang: 18 S.
• Fussnoten: Gesehen am 28.07.2017
• Titel Quelle: Enthalten in: PeerJ
• Ort Quelle: London [u.a.] : PeerJ, Inc., 2013
• Jahr Quelle: 2017
• Band/Heft Quelle: 5(2017) Artikel-Nummer e3357, 18 Seiten
• ISSN Quelle: 2167-8359
• DOI: doi:10.7717/peerj.3357
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1561315737
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Cucurbitacins, a class of toxic tetracyclic triterpenoids in Cucurbitaceae, modulate many molecular targets. Here we investigated the interactions of cucurbitacin B, E and I with cytoskeletal proteins such as microtubule and actin filaments. The effects of cucurbitacin B, E and I on microtubules and actin filaments were studied in living cells (Hela and U2OS) and in vitro using GFP markers, immunofluorescence staining and in vitro tubulin polymerization assay. Cucurbitacin B, E and I apparently affected microtubule structures in living cells and cucurbitacin E inhibited tubulin polymerization in vitro with IC50 value of 566.91 ± 113.5 µM. Cucurbitacin E did not affect the nucleation but inhibited the growth phase and steady state during microtubule assembly in vitro. In addition, cucurbitacin B, E and I all altered mitotic spindles and induced the cell cycle arrest at G2/M phase. Moreover, they all showed potent effects on actin cytoskeleton by affecting actin filaments through the depolymerization and aggregation. The interactions of cucubitacin B, E and I with microtubules and actin filaments present new insights into their modes of action.