Essential role of the V-ATPase in male gametophyte development

• Verfasst von: Dettmer, Jan [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Titel: Essential role of the V-ATPase in male gametophyte development
• Verf.angabe: Jan Dettmer, Daniel Schubert, Olga Calvo-Weimar, York-Dieter Stierhof, Renate Schmidt and Karin Schumacher
• Jahr: 2005
• Umfang: 8 S.
• Teil: volume:41
• Teil: year:2005
• Teil: number:1
• Teil: pages:117-124
• Teil: extent:8
• Fussnoten: Gesehen am 01.08.2017
• Titel Quelle: Enthalten in: The plant journal
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1991
• Jahr Quelle: 2005
• Band/Heft Quelle: 41(2005), 1, Seite 117-124
• ISSN Quelle: 1365-313X
• DOI: doi:10.1111/j.1365-313X.2004.02282.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Arabidopsis
• Sach-SW: gametogenesis
• Sach-SW: Golgi
• Sach-SW: V-ATPase
• K10plus-PPN: 1561441473

Abstract

Intracellular pH homeostasis is a prerequisite for biological processes and requires the action of proton pumps. The vacuolar H+-ATPase (V-ATPase) is involved in regulating pH in endomembrane compartments of all eukaryotic cells. In plants, there is an additional endomembrane proton pump, H+-pyrophosphatase (H+-PPase). However, the relative roles of the two types of pumps in endomembrane acidification and energization of secondary active transport are unclear. Here, we show that a strong T-DNA insertion allele of VHA-A, the single copy gene encoding the catalytic subunit of the Arabidopsis V-ATPase, causes complete male and partial female gametophytic lethality. Severe changes in the morphology of Golgi stacks and Golgi-derived vesicles in male gametophytes are the first visible symptoms of cell degeneration leading to a failure to develop mature pollen. Similar effects on Golgi morphology were observed in pollen tubes when growth was blocked by Concanamycin A, a specific V-ATPase inhibitor. Taken together, our results suggests that V-ATPase function is essential for Golgi organization and development of the male gametophyte.