Arabidopsis vacuolar H+-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis

• Verfasst von: Strompen, Georg [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Titel: Arabidopsis vacuolar H+-ATPase subunit E isoform 1 is required for Golgi organization and vacuole function in embryogenesis
• Verf.angabe: Georg Strompen, Jan Dettmer, York-Dieter Stierhof, Karin Schumacher, Gerd Jürgens and Ulrike Mayer
• Jahr des Originals: 2004
• Umfang: 8 S.
• Fussnoten: First published: 17 November 2004 ; Gesehen am 09.08.2017 ; Im Titel ist „+“ hochgestellt
• Titel Quelle: Enthalten in: The plant journal
• Jahr Quelle: 2005
• Band/Heft Quelle: 41(2005), 1, S. 125-132
• ISSN Quelle: 1365-313X
• DOI: doi:10.1111/j.1365-313X.2004.02283.x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1562271180

Abstract

Vacuolar H+-ATPases play an important role in maintaining the pH of endomembrane compartments in eukaryotic cells. The functional relevance of this homeostasis for multicellular development has not been studied in plants. Here, we analyze the biological consequences resulting from the lack of subunit E isoform 1 (VHA-E1) encoded by the Arabidopsis TUFF gene. tuff mutant embryos are lethal, displaying variably enlarged cells with multiple nuclei, large vacuoles containing inclusions, abnormal organization of Golgi stacks, and cell wall defects. Rescue of embryo lethality by cell cycle-regulated expression of VHA-E1 results in abnormal seedlings with non-functional meristems and defective cell differentiation. VHA-E1 is the predominant isoform in embryogenesis whereas VHA-E3 is expressed mainly in the endosperm and surrounding maternal tissues during seed development, and VHA-E2 is pollen-specific. VHA-E1 protein accumulates at endomembrane compartments including vacuoles and endosomes, but appears absent from the plasma membrane. Our results suggest an essential role for VHA-E1 in maintaining a functional secretory system during somatic development but not in the haploid gametophytes.