Tracking protein turnover and degradation by microscopy

• Verfasst von: Knop, Michael [VerfasserIn]
• Verfasst von: Edgar, Bruce [VerfasserIn]
• Titel: Tracking protein turnover and degradation by microscopy
• Titelzusatz: photo-switchable versus time-encoded fluorescent proteins
• Verf.angabe: Michael Knop and Bruce A. Edgar
• E-Jahr: 2014
• Jahr: 2014 Apr 16
• Umfang: $y4(2014) Artikel-Nummer 140002, 4 Seiten S.
• Fussnoten: Gesehen am 09.08.2017
• Titel Quelle: Enthalten in: Open biology
• Ort Quelle: London : Royal Society Publishing, 2010
• Jahr Quelle: 2014
• Band/Heft Quelle: 4(2014), Seite ?
• ISSN Quelle: 2046-2441
• DOI: doi:10.1098/rsob.140002
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Zebrafish
• Sach-SW: Animals
• Sach-SW: fluorescent timer proteins
• Sach-SW: live cell microscopy
• Sach-SW: Luminescent Proteins
• Sach-SW: Microscopy, Confocal
• Sach-SW: photo-switchable fluorescent proteins
• Sach-SW: protein dynamics
• Sach-SW: Protein Isoforms
• Sach-SW: Receptors, G-Protein-Coupled
• Sach-SW: Recombinant Fusion Proteins
• K10plus-PPN: 1562279300

Abstract

Expanded fluorescent protein techniques employing photo-switchable and fluorescent timer proteins have become important tools in biological research. These tools allow researchers to address a major challenge in cell and developmental biology, namely obtaining kinetic information about the processes that determine the distribution and abundance of proteins in cells and tissues. This knowledge is often essential for the comprehensive understanding of a biological process, and/or required to determine the precise point of interference following an experimental perturbation.