Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation

• Verfasst von: Erhardt, Sylvia [VerfasserIn]
• Verfasst von: Mellone, Barbara G. [VerfasserIn]
• Verfasst von: Betts, Craig M. [VerfasserIn]
• Verfasst von: Zhang, Weiguo [VerfasserIn]
• Verfasst von: Karpen, Gary H. [VerfasserIn]
• Verfasst von: Straight, Aaron F. [VerfasserIn]
• Titel: Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation
• Verf.angabe: Sylvia Erhardt, Barbara G. Mellone, Craig M. Betts, Weiguo Zhang, Gary H. Karpen, and Aaron F. Straight
• E-Jahr: 2008
• Jahr: December 1, 2008
• Umfang: 14 S.
• Fussnoten: Gesehen am 15.08.2017
• Titel Quelle: Enthalten in: The journal of cell biology
• Ort Quelle: New York, NY : Rockefeller Univ. Press, 1962
• Jahr Quelle: 2008
• Band/Heft Quelle: 183(2008), 5, Seite 805-818
• ISSN Quelle: 1540-8140
• DOI: doi:10.1083/jcb.200806038
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1562451936

Abstract

Centromeres are the structural and functional foundation for kinetochore formation, spindle attachment, and chromosome segregation. In this study, we isolated factors required for centromere propagation using genome-wide RNA interference screening for defects in centromere protein A (CENP-A; centromere identifier [CID]) localization in Drosophila melanogaster. We identified the proteins CAL1 and CENP-C as essential factors for CID assembly at the centromere. CID, CAL1, and CENP-C coimmunoprecipitate and are mutually dependent for centromere localization and function. We also identified the mitotic cyclin A (CYCA) and the anaphase-promoting complex (APC) inhibitor RCA1/Emi1 as regulators of centromere propagation. We show that CYCA is centromere localized and that CYCA and RCA1/Emi1 couple centromere assembly to the cell cycle through regulation of the fizzy-related/CDH1 subunit of the APC. Our findings identify essential components of the epigenetic machinery that ensures proper specification and propagation of the centromere and suggest a mechanism for coordinating centromere inheritance with cell division.