Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Knop, Michael [VerfasserIn]   i
Titel:Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst vomplex and the rxocytic SNARE machinery in yeast
Verf.angabe:Michael Knop, K. Juha Miller, Massimiliano Mazza, DeJiang Feng, Marion Weber, Sirkka Keränen, and Jussi Jäntti
Umfang:14 S.
Fussnoten:Gesehen am 21.08.2017
Titel Quelle:Enthalten in: Molecular biology of the cell
Jahr Quelle:2005
Band/Heft Quelle:16(2005), 10, S. 4543-4556
ISSN Quelle:1939-4586
Abstract:In this study, we have analyzed the association of the Sec1p interacting protein Mso1p with the membrane fusion machinery in yeast. We show that Mso1p is essential for vesicle fusion during prospore membrane formation. Green fluorescent protein-tagged Mso1p localizes to the sites of exocytosis and at the site of prospore membrane formation. In vivo and in vitro experiments identified a short amino-terminal sequence in Mso1p that mediates its interaction with Sec1p and is needed for vesicle fusion. A point mutation, T47A, within the Sec1p-binding domain abolishes Mso1p functionality in vivo, and mso1T47A mutant cells display specific genetic interactions with sec1 mutants. Mso1p coimmunoprecipitates with Sec1p, Sso1/2p, Snc1/2p, Sec9p, and the exocyst complex subunit Sec15p. In sec4-8 and SEC4I133 mutant cells, association of Mso1p with Sso1/2p, Snc1/2p, and Sec9p is affected, whereas interaction with Sec1p persists. Furthermore, in SEC4I133 cells the dominant negative Sec4I133p coimmunoprecipitates with Mso1p-Sec1p complex. Finally, we identify Mso1p as a homologue of the PTB binding domain of the mammalian Sec1p binding Mint proteins. These results position Mso1p in the interface of the exocyst complex, Sec4p, and the SNARE machinery, and reveal a novel layer of molecular conservation in the exocytosis machinery.
DOI:doi:10.1091/mbc.E05-03-0243
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Verlag: http://dx.doi.org/10.1091/mbc.E05-03-0243
 Verlag: http://www.molbiolcell.org/content/16/10/4543
 DOI: https://doi.org/10.1091/mbc.E05-03-0243
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1562586572
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68152181   QR-Code
zum Seitenanfang