AtSTP6, a new pollen-specific H+-monosaccharide symporter from Arabidopsis

• Verfasst von: Scholz-Starke, Joachim [VerfasserIn]
• Verfasst von: Büttner, Michael [VerfasserIn]
• Verfasst von: Sauer, Norbert [VerfasserIn]
• Titel: AtSTP6, a new pollen-specific H+-monosaccharide symporter from Arabidopsis
• Verf.angabe: Joachim Scholz-Starke, Michael Büttner, and Norbert Sauer
• E-Jahr: 2003
• Jahr: January 2003
• Umfang: 8 S.
• Fussnoten: Im Titel ist "+" hochgestellt ; Gesehen am 24.08.2017
• Titel Quelle: Enthalten in: Plant physiology
• Ort Quelle: Oxford : Oxford University Press, 1926
• Jahr Quelle: 2003
• Band/Heft Quelle: 131(2003), 1, Seite 70-77
• ISSN Quelle: 1532-2548
• DOI: doi:10.1104/pp.012666
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1562690213

Abstract

This paper describes the molecular, kinetic, and physiological characterization of AtSTP6, a new member of the Arabidopsis H+/monosaccharide transporter family. The AtSTP6 gene (At3g05960) is interrupted by two introns and encodes a protein of 507 amino acids containing 12 putative transmembrane helices. Expression in yeast (Saccharomyces cerevisiae) shows that AtSTP6 is a high-affinity (K m = 20 μm), broad-spectrum, and uncoupler-sensitive monosaccharide transporter that is targeted to the plasma membrane and that can complement a growth deficiency resulting from the disruption of most yeast hexose transporter genes. Analyses ofAtSTP6-promoter::GUS plants and in situ hybridization experiments detected AtSTP6expression only during the late stages of pollen development. A transposon-tagged Arabidopsis mutant was isolated and homozygous plants were analyzed for potential effects of the Atstp6mutation on pollen viability, pollen germination, fertilization, and seed production. However, differences between wild-type and mutant plants could not be observed.