The cis‐regulatory code of Hox function in Drosophila

• Verfasst von: Sorge, Sebastian [VerfasserIn]
• Verfasst von: Ha, Nati [VerfasserIn]
• Verfasst von: Polychronidou, Maria [VerfasserIn]
• Verfasst von: Friedrich, Jana [VerfasserIn]
• Verfasst von: Kaspar, Petra [VerfasserIn]
• Verfasst von: Mundorf, Juliane [VerfasserIn]
• Verfasst von: Rätzer, Jenny [VerfasserIn]
• Verfasst von: Papagiannouli, Fani [VerfasserIn]
• Verfasst von: Lohmann, Ingrid [VerfasserIn]
• Titel: The cis‐regulatory code of Hox function in Drosophila
• Verf.angabe: Sebastian Sorge, Nati Ha, Maria Polychronidou, Jana Friedrich, Daniela Bezdan, Petra Kaspar, Martin H. Schaefer, Stephan Ossowski, Stefan R. Henz, Juliane Mundorf, Jenny Rätzer, Fani Papagiannouli and Ingrid Lohmann
• E-Jahr: 2012
• Jahr: 10 July 2012
• Umfang: 11 S.
• Fussnoten: Gesehen am 24.08.2017
• Titel Quelle: Enthalten in: European Molecular Biology OrganizationThe EMBO journal
• Ort Quelle: [London] : Nature Publishing Group UK, 1982
• Jahr Quelle: 2012
• Band/Heft Quelle: 31(2012), 15, Seite 3323-3333
• ISSN Quelle: 1460-2075
• DOI: doi:10.1038/emboj.2012.179
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Errata: Sorge, Sebastian: Corrigendum
• Sach-SW: ChIP‐seq
• Sach-SW: Deformed
• Sach-SW: Drosophila
• Sach-SW: Hox
• Sach-SW: transcriptional gene regulation
• K10plus-PPN: 1562702459

Abstract

Precise gene expression is a fundamental aspect of organismal function and depends on the combinatorial interplay of transcription factors (TFs) with cis‐regulatory DNA elements. While much is known about TF function in general, our understanding of their cell type‐specific activities is still poor. To address how widely expressed transcriptional regulators modulate downstream gene activity with high cellular specificity, we have identified binding regions for the Hox TF Deformed (Dfd) in the Drosophila genome. Our analysis of architectural features within Hox cis‐regulatory response elements (HREs) shows that HRE structure is essential for cell type‐specific gene expression. We also find that Dfd and Ultrabithorax (Ubx), another Hox TF specifying different morphological traits, interact with non‐overlapping regions in vivo, despite their similar DNA binding preferences. While Dfd and Ubx HREs exhibit comparable design principles, their motif compositions and motif‐pair associations are distinct, explaining the highly selective interaction of these Hox proteins with the regulatory environment. Thus, our results uncover the regulatory code imprinted in Hox enhancers and elucidate the mechanisms underlying functional specificity of TFs in vivo.