Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| Online-Ressource |
Verfasst von: | Geißler, Silke [VerfasserIn]  |
| Pereira, Gislene [VerfasserIn]  |
| Knop, Michael [VerfasserIn]  |
| Schiebel, Elmar [VerfasserIn]  |
Titel: | The spindle pole body component Spc98p interacts with the γ‐tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment |
Verf.angabe: | Silke Geissler, Gislene Pereira, Anne Spang, Michael Knop, Sylvie Souès, John Kilmartin and Elmar Schiebel |
Umfang: | 13 S. |
Fussnoten: | Gesehen am 31.08.2017 |
Titel Quelle: | Enthalten in: European Molecular Biology Organization: The EMBO journal |
Jahr Quelle: | 1996 |
Band/Heft Quelle: | 15(1996), 15, S. 3899-3911 |
ISSN Quelle: | 1460-2075 |
Abstract: | Tub4p is a novel tubulin found in Saccharomyces cerevisiae. It most resembles gamma-tubulin and, like it, is localized to the yeast microtubule organizing centre, the spindle pole body (SPB). In this paper we report the identification of SPC98 as a dosage-dependent suppressor of the conditional lethal tub4-1 allele. SPC98 encodes an SPB component of 98 kDa which is identical to the previously described 90 kDa SPB protein. Strong overexpression of SPC98 is toxic, causing cells to arrest with a large bud, defective microtubule structures, undivided nucleus and replicated DNA. The toxicity of SPC98 overexpression was relieved by co-overexpression of TUB4. Further evidence for an interaction between Tub4p and Spc98p came from the synthetic toxicity of tub4-1 and spc98-1 alleles, the dosage-dependent suppression of spc98-4 by TUB4, the binding of Tub4p to Spc98p in the two-hybrid system and the co-immunoprecipitation of Tub4p and Spc98p. In addition, Spc98-1p is defective in its interaction with Tub4p in the two-hybrid system. We suggest a model in which Tub4p and Spc98p form a complex involved in microtubule organization by the SPB. |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Verlag: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC452092/ |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1562960083 |
Verknüpfungen: | → Zeitschrift |
¬The¬ spindle pole body component Spc98p interacts with the γ‐tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment / Geißler, Silke [VerfasserIn] (Online-Ressource)
68155716