Rapid detection of cefotaxime-resistant Escherichia coli by LC-MS

• Verfasst von: Burrer, Achim [VerfasserIn]
• Verfasst von: Findeisen, Peter [VerfasserIn]
• Verfasst von: Grundt, Alexander [VerfasserIn]
• Verfasst von: Miethke, Thomas [VerfasserIn]
• Verfasst von: Neumaier, Michael [VerfasserIn]
• Titel: Rapid detection of cefotaxime-resistant Escherichia coli by LC-MS
• Verf.angabe: A. Burrer, P. Findeisen, E. Jäger, B. Ghebremedhin, A. Grundt, P. Ahmad-Nejad, T. Miethke, M. Neumaier
• E-Jahr: 2015
• Jahr: December 2015
• Umfang: 5 S.
• Fussnoten: Gesehen am 06.09.2017
• Titel Quelle: Enthalten in: International journal of medical microbiology
• Ort Quelle: München : Elsevier, 2000
• Jahr Quelle: 2015
• Band/Heft Quelle: 305(2015), 8, Seite 860-864
• ISSN Quelle: 1618-0607
• DOI: doi:10.1016/j.ijmm.2015.08.004
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Antibiotic susceptibility testing
• Sach-SW: Beta-lactamase
• Sach-SW: Mass spectrometry
• K10plus-PPN: 1563267055

Abstract

Antibiotic resistance is an unsolved healthcare problem with increasing impact on patient management in the last years. In particular, multidrug resistance among Gram-negative bacterial strains has become the most pressing challenge. In order to deliver the most efficacious antimicrobial therapy with minimum delay, rapid diagnostic tests are required in order to detect multidrug resistant pathogens early during infection. In line with these efforts, we have developed a mass spectrometry-based assay for the rapid determination of ampicillin and cefotaxime resistance. The assay quantifies beta-lactamase activities towards ampicillin and cefotaxime within a turnaround time of 150min, which is substantially faster than classical susceptibility testing.