| |  Online-Ressource |
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| Verfasst von: | Schrenk-Siemens, Katrin [VerfasserIn]  |
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| | Wende, Hagen [VerfasserIn] |
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| | Prato, Vincenzo [VerfasserIn] |
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| | Song, Kun [VerfasserIn] |
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| | Rostock, Charlotte [VerfasserIn] |
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| | Utikal, Jochen [VerfasserIn] |
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| | Lechner, Stefan [VerfasserIn] |
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| | Siemens, Jan [VerfasserIn] |
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| Titel: | PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors |
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| Verf.angabe: | Katrin Schrenk-Siemens, Hagen Wende, Vincenzo Prato, Kun Song, Charlotte Rostock, Alexander Loewer, Jochen Utikal, Gary R. Lewin, Stefan G. Lechner and Jan Siemens |
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| Jahr: | 2015 |
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| Umfang: | 7 S. |
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| Fussnoten: | Published: 3 December 2014 ; Gesehen am 06.09.2017 |
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| Titel Quelle: | Enthalten in: Nature neuroscience |
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| Ort Quelle: | New York, NY : Nature America, 1998 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 18(2015), 1, Seite 10-16 |
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| ISSN Quelle: | 1546-1726 |
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| Abstract: | Human sensory neurons are inaccessible for functional examination, and thus little is known about the mechanisms mediating touch sensation in humans. Here we demonstrate that the mechanosensitivity of human embryonic stem (hES) cell-derived touch receptors depends on PIEZO2. To recapitulate sensory neuron development in vitro, we established a multistep differentiation protocol and generated sensory neurons via the intermediate production of neural crest cells derived from hES cells or human induced pluripotent stem (hiPS) cells. The generated neurons express a distinct set of touch receptor-specific genes and convert mechanical stimuli into electrical signals, their most salient characteristic in vivo. Strikingly, mechanosensitivity is lost after CRISPR/Cas9-mediated PIEZO2 gene deletion. Our work establishes a model system that resembles human touch receptors, which may facilitate mechanistic analysis of other sensory subtypes and provide insight into developmental programs underlying sensory neuron diversity. |
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| DOI: | doi:10.1038/nn.3894 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1038/nn.3894 |
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| | Volltext: http://www.nature.com.ezproxy.medma.uni-heidelberg.de/neuro/journal/v18/n1/full/nn.3894.html?foxtrotcallback=true |
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| | DOI: https://doi.org/10.1038/nn.3894 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cellular neuroscience |
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| | Mechanotransduction |
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| | Neural stem cells |
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| K10plus-PPN: | 1563269279 |
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| Verknüpfungen: | → Zeitschrift |
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PIEZO2 is required for mechanotransduction in human stem cell-derived touch receptors / Schrenk-Siemens, Katrin [VerfasserIn]; 2015 (Online-Ressource)
