Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry

• Verfasst von: Schwend, Thomas [VerfasserIn]
• Verfasst von: Ruppert, Thomas [VerfasserIn]
• Verfasst von: Szenthe, Andrea [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry
• Verf.angabe: Thomas Schwend, Inga Redwanz, Thomas Ruppert, Andrea Szenthe, Michael Wink
• Umfang: 6 S.
• Fussnoten: Gesehen am 04.10.2017
• Titel Quelle: Enthalten in: Journal of chromatography / A
• Jahr Quelle: 2003
• Band/Heft Quelle: 1009(2003), 1, S. 105-110
• ISSN Quelle: 1873-3778
• DOI: doi:10.1016/S0021-9673(03)00622-8
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1564049663

Abstract

Lupinus albus cell cultures secrete a large set of hydrolases into their medium with a small number of highly abundant proteins. We have investigated the protein composition of the medium with two different methods, two-dimensional gel electrophoresis-electrospray ionisation tandem mass spectrometry (ESI-MS-MS) and enzymatic analysis. The proteomic approach revealed the presence of several abundant proteins that had been overlooked using standard enzyme assays, e.g. subtilisin-like protease, glucan 1,3-β-glucosidase, α-amylase, chitinase, thaumatin-like protein, and a secretory pathogenesis-related protein. Several low-abundant proteins were readily detectable by enzymatic assays (peroxidases, phosphatase), but could not be found by ESI-MS-MS. Both data sets support the assumed lytic function of the medium, which appears to be similar to that of the plant vacuole.