Tissue factor-dependent pathway is not involved in exercise-induced formation of thrombin and fibrin

• Verfasst von: Weiss, Claus [VerfasserIn]
• Verfasst von: Bierhaus, Angelika [VerfasserIn]
• Verfasst von: Kinscherf, Ralf [VerfasserIn]
• Verfasst von: Hack, Volker [VerfasserIn]
• Verfasst von: Nawroth, Peter Paul [VerfasserIn]
• Verfasst von: Bärtsch, Peter [VerfasserIn]
• Titel: Tissue factor-dependent pathway is not involved in exercise-induced formation of thrombin and fibrin
• Verf.angabe: Claus Weiss, Angelika Bierhaus, Ralf Kinscherf, Volker Hack, Thomas Luther, Peter Paul Nawroth, and Peter Bärtsch
• Umfang: 8 S.
• Fussnoten: Vom Autor zitiert als: The tissue factor dependent pathway is not involved in exercise-induced activation of coagulation in PBMC ; Gesehen am 20.10.2017
• Titel Quelle: Enthalten in: Journal of applied physiology
• Jahr Quelle: 2002
• Band/Heft Quelle: 92(2002), 1, S. 211-218
• ISSN Quelle: 1522-1601
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1564633039

Abstract

In healthy individuals, prolonged intensive physical exercise leads to an activation of blood coagulation that results in the formation of thrombin and fibrin. This study investigated whether oxidative stress during intensive physical exercise induces tissue factor (TF) via activation of the redox-responsive transcription factor nuclear factor-κB (NF-κB). Twelve young men performed a standardized 1-h maximal run on a treadmill that gave rise to significant increases of markers of thrombin and fibrin formation. The ratio of intracellular reduced to oxidized glutathione as measured by HPLC decreased from 23.3 ± 10.7 to 14.2 ± 6.5 (P < 0.05), indicating the generation of free radicals during exercise. Electrophoretic mobility shift assays from nuclear extracts of peripheral blood mononuclear cells revealed that exercise testing increased NF-κB (p50/p65) binding activity to a NF-κB consensus sequence by 105 ± 68% (P < 0.01) but did not affect NF-κB (p65/c-Rel) binding to a nonconsensus-κB-like site present in the TF promoter. Consistently, there was no exercise-induced increase in TF expression as demonstrated by TF-specific immunofluorescence staining and ELISA. Thus selective activation of NF-κB (p50/p65) during intensive physical exercise does not result in the expression of TF, suggesting that the TF-dependent pathway in peripheral blood mononuclear cells does not account for exercise-induced formation of thrombin and fibrin.