Increased cyclooxygenase-2 expression in peripheral blood mononuclear cells of smokers and hyperlipidemic subjects

• Verfasst von: Bonaterra, Gabriel [VerfasserIn]
• Verfasst von: Hildebrandt, Wulf [VerfasserIn]
• Verfasst von: Bodens, Anne [VerfasserIn]
• Verfasst von: Sauer, Roland [VerfasserIn]
• Verfasst von: Dugi, Klaus A. [VerfasserIn]
• Verfasst von: Deigner, Hans-Peter [VerfasserIn]
• Verfasst von: Dröge, Wulf [VerfasserIn]
• Verfasst von: Metz, Jürgen [VerfasserIn]
• Verfasst von: Kinscherf, Ralf [VerfasserIn]
• Titel: Increased cyclooxygenase-2 expression in peripheral blood mononuclear cells of smokers and hyperlipidemic subjects
• Verf.angabe: Gabriel A. Bonaterra, Wulf Hildebrandt, Anne Bodens, Roland Sauer, Klaus A. Dugi, Hans-Peter Deigner, Wulf Dröge, Jürgen Metz, Ralf Kinscherf
• Jahr des Originals: 2004
• Umfang: 8 S.
• Fussnoten: Available online: 11 November 2004 ; Gesehen am 23.10.2017
• Titel Quelle: Enthalten in: Free radical biology and medicine
• Jahr Quelle: 2005
• Band/Heft Quelle: 38(2005), 2, S. 235-242
• ISSN Quelle: 1873-4596
• DOI: doi:10.1016/j.freeradbiomed.2004.10.021
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1564642593

Abstract

Cyclooxygenase (COX)-2 is expressed in macrophages of arteriosclerotic lesions and promotes inflammation. We investigated whether COX-2 is already expressed in peripheral blood mononuclear cells (PBMCs) of subjects possessing risk-related factors, such as in smokers and hyperlipidemics. PBMCs were isolated from the venous blood of normolipidemic nonsmokers (NL-NSM; n = 15), normolipidemic smokers (NL-SM; n = 12), hyperlipidemic nonsmokers (HL-NSM; n = 10), and hyperlipidemic smokers (HL-SM; n = 10). RNA from PBMCs was used for RT-PCR. Plasma concentrations of oxidized low-density lipoproteins (oxLDL) were measured by ELISA, those of glutamate and cystine by HPLC. The results show that COX-2 expression in PBMCs was significantly increased in the groups with cardiovascular risk factors (NL-SM, HL-SM, HL-NSM) compared with NL-NSM. COX-2 expression in PBMCs was positively correlated with concentrations of total serum cholesterol, oxLDL, glutamate, or cystine. We suggest that the elevated COX-2 expression indicates a priming of PBMCs as a response to a systemic pro-oxidative and proinflammatory shift in subjects with cardiovascular risk factors, which might also contribute to growth and instability of arteriosclerotic lesions.