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Status: Bibliographieeintrag

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Verfasst von:Zitzmann, Sabine [VerfasserIn]   i
 Mier, Walter [VerfasserIn]   i
 Schad, Arno [VerfasserIn]   i
 Kinscherf, Ralf [VerfasserIn]   i
 Askoxylakis, Vasileios [VerfasserIn]   i
 Krämer, Susanne [VerfasserIn]   i
 Altmann, Annette [VerfasserIn]   i
 Eisenhut, Michael [VerfasserIn]   i
 Haberkorn, Uwe [VerfasserIn]   i
Titel:A new prostate carcinoma binding peptide (DUP-1) for tumor imaging and therapy
Verf.angabe:Sabine Zitzmann, Walter Mier, Arno Schad, Ralf Kinscherf, Vasileios Askoxylakis, Susanne Krämer, Annette Altmann, Michael Eisenhut, Uwe Haberkorn
E-Jahr:2005
Jahr:January 1, 2005
Umfang:8 S.
Fussnoten:Gesehen am 23.10.2017
Titel Quelle:Enthalten in: Clinical cancer research
Ort Quelle:Philadelphia, Pa. [u.a.] : AACR, 1995
Jahr Quelle:2005
Band/Heft Quelle:11(2005), 1, Seite 139-146
ISSN Quelle:1557-3265
Abstract:PURPOSE: Prostate carcinomas belong to the most widespread tumors, and their number is increasing. Imaging modalities used for diagnosis, such as ultrasound, computed tomography, and positron emission tomography, often produce poor results. Radiolabeled peptides with high sensitivity and specificity for prostate cancer would be a desirable tool for tumor diagnosis and treatment. EXPERIMENTAL DESIGN: We used phage display and the prostate-specific membrane antigen-negative cell line DU-145 to identify a peptide. The isolated DUP-1 was tested in vitro for its binding specificity, kinetics, and affinity. Internalization of the peptide was evaluated with confocal microscopy. The tumor accumulation in a nude mouse model was analyzed with 131I-labeled DUP-1 in PC-3 and DU-145 prostate tumors as well as in the rat prostate tumor model AT-1. RESULTS: The synthesized peptide showed rapid binding kinetics peaking at 10 minutes. It shows specific binding to prostate carcinoma cells but low binding affinity to nontumor cells. Peptide binding is competed with unlabeled DUP-1, and a time-dependent internalization into DU-145 cells was shown. Biodistribution studies of DUP-1 in nude mice with s.c. transplanted DU-145 and PC-3 tumors showed a tumor accumulation of 5% and 7% injected dose per gram, and bound peptide could not be removed by perfusion. The rat prostate tumor model showed an increase of radioactivity in the prostate tumor up to 300% in comparison with normal prostate tissue. CONCLUSIONS: DUP-1 holds promise as a lead peptide structure applicable in the development of new diagnostic tracers or anticancer agents that specifically target prostate carcinoma.
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Kostenfrei: Volltext: http://clincancerres.aacrjournals.org/content/11/1/139.long
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cell Line, Tumor
 Dose-Response Relationship, Drug
 Humans
 Animals
 Microscopy, Confocal
 Protein Binding
 Male
 Neoplasms
 Neoplasm Transplantation
 Rats
 Antigens, Neoplasm
 Fluorescein-5-isothiocyanate
 Kinetics
 Mice
 Mice, Nude
 Microscopy, Fluorescence
 Peptides
 Prostatic Neoplasms
 Sensitivity and Specificity
 Time Factors
K10plus-PPN:1564643751
Verknüpfungen:→ Zeitschrift

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