| |  Online-Ressource |
|---|
| Verfasst von: | Möller, Kerstin [VerfasserIn]  |
|---|
| | Adolph, Oliver [VerfasserIn] |
|---|
| | Grünow, Jennifer [VerfasserIn] |
|---|
| | Elrod, Julia [VerfasserIn] |
|---|
| | Popa, Mirunda [VerfasserIn] |
|---|
| | Ghosh, Subhajit [VerfasserIn] |
|---|
| | Schwarz, Manuel [VerfasserIn] |
|---|
| | Schwale, Chrysovalandis [VerfasserIn] |
|---|
| | Grässle, Sandra [VerfasserIn] |
|---|
| | Huck, Volker [VerfasserIn] |
|---|
| | Bruehl, Claus [VerfasserIn] |
|---|
| | Wieland, Thomas [VerfasserIn] |
|---|
| | Schneider, Stefan W. [VerfasserIn] |
|---|
| | Nobiling, Rainer [VerfasserIn] |
|---|
| | Wagner, Andreas H. [VerfasserIn] |
|---|
| | Hecker, Markus [VerfasserIn] |
|---|
| Titel: | Mechanism and functional impact of CD40 ligand-induced von Willebrand factor release from endothelial cells |
|---|
| Verf.angabe: | K. Möller, O. Adolph, J. Grünow, J. Elrod, M. Popa, S. Ghosh, M. Schwarz, C. Schwale, S. Grässle, V. Huck, C. Bruehl, T. Wieland, S.W. Schneider, R. Nobiling, A.H. Wagner, M. Hecker |
|---|
| Jahr: | 2015 |
|---|
| Umfang: | 14 S. |
|---|
| Fussnoten: | Gesehen am 11.12.2017 |
|---|
| Titel Quelle: | Enthalten in: Thrombosis and haemostasis |
|---|
| Ort Quelle: | Stuttgart : Thieme, 1976 |
|---|
| Jahr Quelle: | 2015 |
|---|
| Band/Heft Quelle: | 113(2015), 5, Seite 1095-1108 |
|---|
| ISSN Quelle: | 2567-689X |
|---|
| Abstract: | Co-stimulation via CD154 binding to CD40, pivotal for both innate and adaptive immunity, may also link haemostasis to vascular remodelling. Here we demonstrate that human platelet-bound or recombinant soluble CD154 (sCD154) elicit the release from and tethering of ultra-large (UL) von Willebrand factor (vWF) multimers to the surface of human cultured endothelial cells (ECs) exposed to shear stress. This CD40-mediated ULVWF multimer release from the Weibel-Palade bodies was triggered by consecutive activation of TRAF6, the tyrosine kinase c-Src and phospholipase Cγ1 followed by inositol-1,4,5 trisphosphate-mediated calcium mobilisation. Subsequent exposure to human washed platelets caused ULVWF multimer-platelet string formation on the EC surface in a shear stress-dependent manner. Platelets tethered to these ULVWF multimers exhibited P-selectin on their surface and captured labelled monocytes from the superfusate. When exposed to shear stress and sCD154, native ECs from wild-type but not CD40 or vWF-deficient mice revealed a comparable release of ULVWF multimers to which murine washed platelets rapidly adhered, turning P-selectin-positive and subsequently capturing monocytes from the perfusate. This novel CD154-provoked ULVWF multimer-platelet string formation at normal to fast flow may contribute to vascular remodelling processes requiring the perivascular or intravascular accumulation of pro-inflammatory macrophages such as arteriogenesis or atherosclerosis. |
|---|
| DOI: | doi:10.1160/TH14-04-0336 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1160/TH14-04-0336 |
|---|
| | Volltext: https://th-schattauer-de.ezproxy.medma.uni-heidelberg.de/contents/archive/issue/2210/manuscript/23911.html |
|---|
| | DOI: https://doi.org/10.1160/TH14-04-0336 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| K10plus-PPN: | 1566208521 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Mechanism and functional impact of CD40 ligand-induced von Willebrand factor release from endothelial cells / Möller, Kerstin [VerfasserIn]; 2015 (Online-Ressource)
