| |  Online-Ressource |
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| Verfasst von: | Möller, Kerstin [VerfasserIn]  |
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| | Adolph, Oliver [VerfasserIn] |
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| | Grünow, Jennifer [VerfasserIn] |
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| | Elrod, Julia [VerfasserIn] |
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| | Popa, Mirunda [VerfasserIn] |
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| | Ghosh, Subhajit [VerfasserIn] |
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| | Schwarz, Manuel [VerfasserIn] |
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| | Schwale, Chrysovalandis [VerfasserIn] |
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| | Grässle, Sandra [VerfasserIn] |
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| | Huck, Volker [VerfasserIn] |
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| | Bruehl, Claus [VerfasserIn] |
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| | Wieland, Thomas [VerfasserIn] |
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| | Schneider, Stefan W. [VerfasserIn] |
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| | Nobiling, Rainer [VerfasserIn] |
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| | Wagner, Andreas H. [VerfasserIn] |
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| | Hecker, Markus [VerfasserIn] |
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| Titel: | Mechanism and functional impact of CD40 ligand-induced von Willebrand factor release from endothelial cells |
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| Verf.angabe: | K. Möller, O. Adolph, J. Grünow, J. Elrod, M. Popa, S. Ghosh, M. Schwarz, C. Schwale, S. Grässle, V. Huck, C. Bruehl, T. Wieland, S.W. Schneider, R. Nobiling, A.H. Wagner, M. Hecker |
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| Jahr: | 2015 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 11.12.2017 |
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| Titel Quelle: | Enthalten in: Thrombosis and haemostasis |
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| Ort Quelle: | Stuttgart : Thieme, 1976 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 113(2015), 5, Seite 1095-1108 |
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| ISSN Quelle: | 2567-689X |
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| Abstract: | Co-stimulation via CD154 binding to CD40, pivotal for both innate and adaptive immunity, may also link haemostasis to vascular remodelling. Here we demonstrate that human platelet-bound or recombinant soluble CD154 (sCD154) elicit the release from and tethering of ultra-large (UL) von Willebrand factor (vWF) multimers to the surface of human cultured endothelial cells (ECs) exposed to shear stress. This CD40-mediated ULVWF multimer release from the Weibel-Palade bodies was triggered by consecutive activation of TRAF6, the tyrosine kinase c-Src and phospholipase Cγ1 followed by inositol-1,4,5 trisphosphate-mediated calcium mobilisation. Subsequent exposure to human washed platelets caused ULVWF multimer-platelet string formation on the EC surface in a shear stress-dependent manner. Platelets tethered to these ULVWF multimers exhibited P-selectin on their surface and captured labelled monocytes from the superfusate. When exposed to shear stress and sCD154, native ECs from wild-type but not CD40 or vWF-deficient mice revealed a comparable release of ULVWF multimers to which murine washed platelets rapidly adhered, turning P-selectin-positive and subsequently capturing monocytes from the perfusate. This novel CD154-provoked ULVWF multimer-platelet string formation at normal to fast flow may contribute to vascular remodelling processes requiring the perivascular or intravascular accumulation of pro-inflammatory macrophages such as arteriogenesis or atherosclerosis. |
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| DOI: | doi:10.1160/TH14-04-0336 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1160/TH14-04-0336 |
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| | Volltext: https://th-schattauer-de.ezproxy.medma.uni-heidelberg.de/contents/archive/issue/2210/manuscript/23911.html |
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| | DOI: https://doi.org/10.1160/TH14-04-0336 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1566208521 |
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| Verknüpfungen: | → Zeitschrift |
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Mechanism and functional impact of CD40 ligand-induced von Willebrand factor release from endothelial cells / Möller, Kerstin [VerfasserIn]; 2015 (Online-Ressource)
