Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors

• Verfasst von: Selhuber-Unkel, Christine [VerfasserIn]
• Verfasst von: Erdmann, Thorsten [VerfasserIn]
• Verfasst von: Schwarz, Ulrich S. [VerfasserIn]
• Titel: Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors
• Verf.angabe: C. Selhuber-Unkel, T. Erdmann, M. López-García, H. Kessler, U.S. Schwarz, and J.P. Spatz
• E-Jahr: 2010
• Jahr: 11 February 2010
• Umfang: 9 S.
• Fussnoten: Gesehen am 08.12.2017
• Titel Quelle: Enthalten in: Biophysical journal
• Ort Quelle: Cambridge, Mass. : Cell Press, 1960
• Jahr Quelle: 2010
• Band/Heft Quelle: 98(2010), 4, Seite 543-551
• ISSN Quelle: 1542-0086
• DOI: doi:10.1016/j.bpj.2009.11.001
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1566179491

Abstract

Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spacings ≥90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings ≤50 nm. Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters. Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength.