Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Gardel, Margaret L. [VerfasserIn]  |
| Sabass, Benedikt [VerfasserIn]  |
| Schwarz, Ulrich S. [VerfasserIn]  |
Titel: | Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed |
Verf.angabe: | Margaret L. Gardel, Benedikt Sabass, Lin Ji, Gaudenz Danuser, Ulrich S. Schwarz, and Clare M. Waterman |
Jahr: | 2008 |
Umfang: | 7 S. |
Fussnoten: | Gesehen am 08.12.2017 |
Titel Quelle: | Enthalten in: The journal of cell biology |
Ort Quelle: | New York, NY : Rockefeller Univ. Press, 1962 |
Jahr Quelle: | 2008 |
Band/Heft Quelle: | 183(2008), 6, Seite 999-1005 |
ISSN Quelle: | 1540-8140 |
Abstract: | How focal adhesions (FAs) convert retrograde filamentous actin (F-actin) flow into traction stress on the extracellular matrix to drive cell migration is unknown. Using combined traction force and fluorescent speckle microscopy, we observed a robust biphasic relationship between F-actin speed and traction force. F-actin speed is inversely related to traction stress near the cell edge where FAs are formed and F-actin motion is rapid. In contrast, larger FAs where the F-actin speed is low are marked by a direct relationship between F-actin speed and traction stress. We found that the biphasic switch is determined by a threshold F-actin speed of 8-10 nm/s, independent of changes in FA protein density, age, stress magnitude, assembly/disassembly status, or subcellular position induced by pleiotropic perturbations to Rho family guanosine triphosphatase signaling and myosin II activity. Thus, F-actin speed is a fundamental regulator of traction force at FAs during cell migration. |
DOI: | doi:10.1083/jcb.200810060 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1083/jcb.200810060 |
| kostenfrei: Volltext: http://jcb.rupress.org/content/183/6/999 |
| DOI: https://doi.org/10.1083/jcb.200810060 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 156618438X |
Verknüpfungen: | → Zeitschrift |
Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed / Gardel, Margaret L. [VerfasserIn]; 2008 (Online-Ressource)
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