Human monocytes downregulate innate response receptors following exposure to the microbial metabolite n-butyrate

• Verfasst von: Lasitschka, Felix [VerfasserIn]
• Verfasst von: Giese, Thomas [VerfasserIn]
• Verfasst von: Paparella, Marco [VerfasserIn]
• Verfasst von: Kurzhals, Stefan [VerfasserIn]
• Verfasst von: Wabnitz, Guido H. [VerfasserIn]
• Verfasst von: Gras, Judith [VerfasserIn]
• Verfasst von: Bode, Konrad A. [VerfasserIn]
• Verfasst von: Heninger, Anne-Kristin [VerfasserIn]
• Verfasst von: Szikszai, Timea [VerfasserIn]
• Verfasst von: Samstag, Yvonne [VerfasserIn]
• Verfasst von: Saeedi, Mohammed al [VerfasserIn]
• Verfasst von: Meuer, Stefan [VerfasserIn]
• Verfasst von: Schröder-Braunstein, Jutta [VerfasserIn]
• Titel: Human monocytes downregulate innate response receptors following exposure to the microbial metabolite n-butyrate
• Verf.angabe: Felix Lasitschka, Thomas Giese, Marco Paparella, Stefan R. Kurzhals, Guido Wabnitz, Katrin Jacob, Judith Gras, Konrad A. Bode, Anne-Kristin Heninger, Timea Sziskzai, Yvonne Samstag, Cornelia Leszinski, Bettina Jocher, Mohammed Al-Saeedi, Stefan C. Meuer, & Jutta Schröder-Braunstein
• E-Jahr: 2017
• Jahr: 6 July 2017
• Umfang: 13 S.
• Fussnoten: Gesehen am 15.12.2017
• Titel Quelle: Enthalten in: Immunity, Inflammation and Disease
• Ort Quelle: Chichester [u.a.] : Wiley, 2013
• Jahr Quelle: 2017
• Band/Heft Quelle: 5(2017), 4, Seite 480-492
• ISSN Quelle: 2050-4527
• DOI: doi:10.1002/iid3.184
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Human
• Sach-SW: inflammation
• Sach-SW: monocytes/macrophages
• Sach-SW: mucosa
• K10plus-PPN: 1566429455
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Introduction: Hyporesponsiveness of human lamina propria immune cells to microbial and nutritional antigens represents one important feature of intestinal homeostasis. It is at least partially mediated by low expression of the innate response receptors CD11b, CD14, CD16 as well as the cystine-glutamate transporter xCT on these cells. Milieu-specific mechanisms leading to the down-regulation of these receptors on circulating monocytes, the precursor cells of resident macrophages, are mostly unknown. Methods: Here, we addressed the question whether the short chain fatty acid n-butyrate, a fermentation product of the mammalian gut microbiota exhibiting histone deacetylase inhibitory activity, is able to modulate expression of these receptors in human circulating monocytes. Results: Exposure to n-butyrate resulted in the downregulation of CD11b, CD14, as well as CD16 surface expression on circulating monocytes. XCT transcript levels in circulating monocytes were also reduced following exposure to n-butyrate. Importantly, treatment resulted in the downregulation of protein and gene expression of the transcription factor PU.1, which was shown to be at least partially required for the expression of CD16 in circulating monocytes. PU.1 expression in resident macrophages in situ was observed to be substantially lower in healthy when compared to inflamed colonic mucosa. Conclusions: In summary, the intestinal microbiota may support symbiosis with the human host organism by n-butyrate mediated downregulation of protein and gene expression of innate response receptors as well as xCT on circulating monocytes following recruitment to the lamina propria. Downregulation of CD16 gene expression may at least partially be caused at the transcriptional level by the n-butyrate mediated decrease in expression of the transcription factor PU.1 in circulating monocytes.