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Verfasst von:Schwarz, Ulrich S. [VerfasserIn]   i
 Alon, Ronen [VerfasserIn]   i
Titel:L-selectin-mediated leukocyte tethering in shear flow is controlled by multiple contacts and cytoskeletal anchorage facilitating fast rebinding events
Verf.angabe:Ulrich S. Schwarz and Ronen Alon
Umfang:6 S.
Fussnoten:Gesehen am 15.12.2017
Titel Quelle:Enthalten in: National Academy of Sciences (Washington, DC): Proceedings of the National Academy of Sciences of the United States of America
Jahr Quelle:2004
Band/Heft Quelle:101(2004), 18, S. 6940-6945
ISSN Quelle:1091-6490
Abstract:L-selectin-mediated tethers result in leukocyte rolling only above a threshold in shear. Here we present biophysical modeling based on recently published data from flow chamber experiments, which supports the interpretation that L-selectin-mediated tethers below the shear threshold correspond to single L-selectin carbohydrate bonds dissociating on the time scale of milliseconds, whereas L-selectin-mediated tethers above the shear threshold are stabilized by multiple bonds and fast rebinding of broken bonds, resulting in tether lifetimes on the time scale of 10-1 seconds. Our calculations for cluster dissociation suggest that the single molecule rebinding rate is of the order of 104 Hz. A similar estimate results if increased tether dissociation for tail-truncated L-selectin mutants above the shear threshold is modeled as diffusive escape of single receptors from the rebinding region due to increased mobility. Using computer simulations, we show that our model yields first-order dissociation kinetics and exponential dependence of tether dissociation rates on shear stress. Our results suggest that multiple contacts, cytoskeletal anchorage of L-selectin, and local rebinding of ligand play important roles in L-selectin tether stabilization and progression of tethers into persistent rolling on endothelial surfaces.
DOI:doi:10.1073/pnas.0305822101
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Verlag: http://dx.doi.org/10.1073/pnas.0305822101
 Verlag: http://www.pnas.org/content/101/18/6940
 DOI: https://doi.org/10.1073/pnas.0305822101
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1566418410
Verknüpfungen:→ Zeitschrift

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