Challenges for super-resolution localization microscopy and biomolecular fluorescent nano-probing in cancer research

• Verfasst von: Hausmann, Michael [VerfasserIn]
• Verfasst von: Pilarczyk, Götz [VerfasserIn]
• Verfasst von: Krufczik, Matthias [VerfasserIn]
• Verfasst von: Hildenbrand, Georg Lars [VerfasserIn]
• Titel: Challenges for super-resolution localization microscopy and biomolecular fluorescent nano-probing in cancer research
• Verf.angabe: Michael Hausmann, Nataša Ilić, Götz Pilarczyk, Jin-Ho Lee, Abiramy Logeswaran, Aurora Paola Borroni, Matthias Krufczik, Franziska Theda, Nadine Waltrich, Felix Bestvater, Georg Hildenbrand, Christoph Cremer and Michael Blank
• E-Jahr: 2017
• Jahr: 28 September 2017
• Fussnoten: Gesehen am 08.01.2018
• Titel Quelle: Enthalten in: International journal of molecular sciences
• Ort Quelle: Basel : Molecular Diversity Preservation International, 2000
• Jahr Quelle: 2017
• Band/Heft Quelle: 18(2017,10) Artikel-Nummer 2066, 21 Seiten
• ISSN Quelle: 1422-0067
• ISSN Quelle: 1661-6596
• DOI: doi:10.3390/ijms18102066
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cancer research
• Sach-SW: chromatin organization
• Sach-SW: chromatin radiation response
• Sach-SW: fluorescent nano-probes
• Sach-SW: receptor conformation changes
• Sach-SW: Smurf2
• Sach-SW: super-resolution localization microscopy
• Sach-SW: γ-H2AX phosphorylation sites
• K10plus-PPN: 1566948398
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Understanding molecular interactions and regulatory mechanisms in tumor initiation, progression, and treatment response are key requirements towards advanced cancer diagnosis and novel treatment procedures in personalized medicine. Beyond decoding the gene expression, malfunctioning and cancer-related epigenetic pathways, investigations of the spatial receptor arrangements in membranes and genome organization in cell nuclei, on the nano-scale, contribute to elucidating complex molecular mechanisms in cells and tissues. By these means, the correlation between cell function and spatial organization of molecules or molecular complexes can be studied, with respect to carcinogenesis, tumor sensitivity or tumor resistance to anticancer therapies, like radiation or antibody treatment. Here, we present several new applications for bio-molecular nano-probes and super-resolution, laser fluorescence localization microscopy and their potential in life sciences, especially in biomedical and cancer research. By means of a tool-box of fluorescent antibodies, green fluorescent protein (GFP) tagging, or specific oligonucleotides, we present tumor relevant re-arrangements of Erb-receptors in membranes, spatial organization of Smad specific ubiquitin protein ligase 2 (Smurf2) in the cytosol, tumor cell characteristic heterochromatin organization, and molecular re-arrangements induced by radiation or antibody treatment. The main purpose of this article is to demonstrate how nano-scaled distance measurements between bio-molecules, tagged by appropriate nano-probes, can be applied to elucidate structures and conformations of molecular complexes which are characteristic of tumorigenesis and treatment responses. These applications open new avenues towards a better interpretation of the spatial organization and treatment responses of functionally relevant molecules, at the single cell level, in normal and cancer cells, offering new potentials for individualized medicine.