Status: Bibliographieeintrag
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| Verfasst von: | Erdmann, Gerrit [VerfasserIn]  |
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| | Horn, Patrick [VerfasserIn] |
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| | Wagner, Wolfgang [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Boutros, Michael [VerfasserIn] |
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| Titel: | Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening |
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| Verf.angabe: | Gerrit Erdmann, Michael Suchanek, Patrick Horn, Fabian Graf, Christian Volz, Thomas Horn, Xian Zhang, Wolfgang Wagner, Anthony D. Ho and Michael Boutros |
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| E-Jahr: | 2015 |
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| Jahr: | 17 May 2015 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 01.02.2018 |
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| Titel Quelle: | Enthalten in: Genome medicine |
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| Ort Quelle: | London : BioMed Central, 2009 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 7(2015) Artikel-Nummer 46, 13 Seiten |
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| ISSN Quelle: | 1756-994X |
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| Abstract: | Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies ranging from tissue repair in regenerative medicine to immunomodulation in graft versus host disease after allogeneic transplantation or in autoimmune diseases. Nonetheless, progress has been hampered by their enormous phenotypic as well as functional heterogeneity and the lack of uniform standards and guidelines for quality control. In this study, we describe a method to perform cellular phenotyping by high-throughput RNA interference in primary human bone marrow MSCs. We have shown that despite heterogeneity of MSC populations, robust functional assays can be established that are suitable for high-throughput and high-content screening. We profiled primary human MSCs against human fibroblasts. Network analysis showed a kinome fingerprint that differs from human primary fibroblasts as well as fibroblast cell lines. In conclusion, this study shows that high-throughput screening in primary human MSCs can be reliably used for kinome fingerprinting. |
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| DOI: | doi:10.1186/s13073-015-0170-2 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1186/s13073-015-0170-2 |
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| | kostenfrei: Volltext: https://doi.org/10.1186/s13073-015-0170-2 |
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| | DOI: https://doi.org/10.1186/s13073-015-0170-2 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell Cycle Profile |
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| | Fibroblast Cell Line |
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| | Human Bone Marrow |
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| | Laser Scanning Cytometry |
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| | Primary Fibroblast |
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| K10plus-PPN: | 1568015410 |
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| Verknüpfungen: | → Zeitschrift |
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Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening / Erdmann, Gerrit [VerfasserIn]; 17 May 2015 (Online-Ressource)
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