Local administration of Mitomycin-C-Treated peripheral blood mononuclear cells (PBMCs) prolongs allograft survival in vascularized composite allotransplantation

• Verfasst von: Radu, Christian Andreas [VerfasserIn]
• Verfasst von: Gebhard, Martha-Maria [VerfasserIn]
• Verfasst von: Germann, Günter [VerfasserIn]
• Verfasst von: Terness, Peter [VerfasserIn]
• Titel: Local administration of Mitomycin-C-Treated peripheral blood mononuclear cells (PBMCs) prolongs allograft survival in vascularized composite allotransplantation
• Verf.angabe: Christian Andreas Radu, Jurij Kiefer, Martha Maria Gebhard, Amir Khosrow Bigdeli, Volker Jürgen Schmidt, Guenter Germann, Marcus Lehnhardt, Peter Terness, Ulrich Kneser, andThomas Kremer
• Jahr: 2016
• Umfang: 9 S.
• Teil: volume:36
• Teil: year:2016
• Teil: number:5
• Teil: pages:417-425
• Teil: extent:9
• Fussnoten: Published online: 17 November 2015 ; Gesehen am 02.02.2018
• Titel Quelle: Enthalten in: Microsurgery
• Ort Quelle: New York, NY [u.a.] : Wiley-Liss, 1979
• Jahr Quelle: 2016
• Band/Heft Quelle: 36(2016), 5, Seite 417-425
• ISSN Quelle: 1098-2752
• DOI: doi:10.1002/micr.30003
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1568033591

Abstract

Background: VCA offers a potential treatment for extensive tissue defects. First results of systemic administration of Mitomycin C-treated PBMCs in VCA demonstrated a significant prolongation of allograft survival. The aim of this study is to evaluate if local administration of MMC-PBMCs prolongs allograft survival in allogeneic hind limb transplantations of the rat. Methods: Sixty allogeneic hind limb transplantations in the rat were performed in six groups. Lewis rats (LEW) were used as hind limb donors and Brown-Norway rats (BN) as recipients. Animals in group A received donor-derived MMC-treated PBMCs locally (i.m.). Group B received no immunosuppressive therapy, group C received a standard immunosuppressive regime consisting of FK506 and Prednisolon, group D (BN to BN) comprised isograft transplantations without immunosuppressive treatment, group E received non-treated PBMCs (i.m.) and group F received phosphate buffered saline (PBS) without cells. The transplanted hind limbs were assessed for color, edema, skin, hair condition, and consistency of the thigh every 8 hours. Results: Rejection in group A was delayed to an average of 7.2 ± 0.6 days. Survival times were significantly prolonged (P < 0.01) compared to control groups B, E, and F (5.5 ± 0.7, 5.8 ± 0.7, and 5.7 ± 0.5 days). Control groups C and D showed no signs of rejection. Conclusion: The findings of this study show that local administration of MMC-PBMCs has no side effects and significantly extends allograft survival. Further experiments with MMC-PBMCs treatments repeated at different time-points and being added to low dose immunosuppressive protocols need to be performed to improve experimental and eventually clinical outcome after VCA. © 2015 Wiley Periodicals, Inc. Microsurgery 36:417-425, 2016.