REPTOR and REPTOR-BP regulate organismal metabolism and transcription downstream of TORC1

• Verfasst von: Tiebe, Marcel [VerfasserIn]
• Verfasst von: Boutros, Michael [VerfasserIn]
• Titel: REPTOR and REPTOR-BP regulate organismal metabolism and transcription downstream of TORC1
• Verf.angabe: Marcel Tiebe, Marilena Lutz, Adriana De La Garza, Tina Buechling, Michael Boutros, and Aurelio A. Teleman
• E-Jahr: 2015
• Jahr: May 4, 2015
• Umfang: 13 S.
• Fussnoten: Gesehen am 05.02.2018
• Titel Quelle: Enthalten in: Developmental cell
• Ort Quelle: Cambridge, Mass. : Cell Press, 2001
• Jahr Quelle: 2015
• Band/Heft Quelle: 33(2015), 3, Seite 272-284
• ISSN Quelle: 1878-1551
• DOI: doi:10.1016/j.devcel.2015.03.013
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1568068956

Abstract

TORC1 regulates growth and metabolism, in part, by influencing transcriptional programs. Here, we identify REPTOR and REPTOR-BP as transcription factors downstream of TORC1 that are required for ∼90% of the transcriptional induction that occurs upon TORC1 inhibition in Drosophila. Thus, REPTOR and REPTOR-BP are major effectors of the transcriptional stress response induced upon TORC1 inhibition, analogous to the role of FOXO downstream of Akt. We find that, when TORC1 is active, it phosphorylates REPTOR on Ser527 and Ser530, leading to REPTOR cytoplasmic retention. Upon TORC1 inhibition, REPTOR becomes dephosphorylated in a PP2A-dependent manner, shuttles into the nucleus, joins its partner REPTOR-BP to bind target genes, and activates their transcription. In vivo functional analysis using knockout flies reveals that REPTOR and REPTOR-BP play critical roles in maintaining energy homeostasis and promoting animal survival upon nutrient restriction.