| |  Online-Ressource |
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| Verfasst von: | Stremmel, Wolfgang [VerfasserIn]  |
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| | Staffer, Simone [VerfasserIn] |
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| | Gan-Schreier, Hongying [VerfasserIn] |
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| | Wannhoff, Andreas [VerfasserIn] |
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| | Bach, Margund [VerfasserIn] |
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| | Gauss, Annika [VerfasserIn] |
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| Titel: | Phosphatidylcholine passes through lateral tight junctions for paracellular transport to the apical side of the polarized intestinal tumor cell-line CaCo2 |
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| Verf.angabe: | Wolfgang Stremmel, Simone Staffer, Hongying Gan-Schreier, Andreas Wannhoff, Margund Bach, Annika Gauss |
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| E-Jahr: | 2016 |
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| Jahr: | September 2016 |
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| Umfang: | 9 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online verfügbar am 27. Juni 2016, Version der Veröffentlichung vom 2. Juli 2016 ; Gesehen am 11.11.2024 |
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| Titel Quelle: | Enthalten in: Biochimica et biophysica acta |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 1947 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 1861(2016), 9, Part A, Seite 1161-1169 |
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| ISSN Quelle: | 1878-2434 |
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| Abstract: | Phosphatidylcholine (PC) is the most abundant phospholipid in intestinal mucus, indicative of a specific transport system across the mucosal epithelium to the intestinal lumen. To elucidate this transport mechanism, we employed a transwell tissue culture system with polarized CaCo2 cells. It was shown that PC could not substantially be internalized by the cells. However, after basal application of increasing PC concentrations, an apical transport of 47.1±6.3nmolh−1mMPC−1 was observed. Equilibrium distribution studies with PC applied in equal concentrations to the basal and apical compartments showed a 1.5-fold accumulation on the expense of basal PC. Disruption of tight junctions (TJ) by acetaldehyde or PPARγ inhibitors or by treatment with siRNA to TJ proteins suppressed paracellular transport by at least 50%. Transport was specific for the choline containing the phospholipids PC, lysoPC and sphingomyelin. We showed that translocation is driven by an electrochemical gradient generated by apical accumulation of Cl− and HCO3− through CFTR. Pretreatment with siRNA to mucin 3 which anchors in the apical plasma membrane of mucosal cells inhibited the final step of luminal PC secretion. PC accumulates in intestinal mucus using a paracellular, apically directed transport route across TJs. |
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| DOI: | doi:10.1016/j.bbalip.2016.06.019 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1016/j.bbalip.2016.06.019 |
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| | kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S1388198116301718 |
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| | Volltext: http://dx.doi.org/10.1016/j.bbalip.2016.06.019 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S1388198116301718 |
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| | DOI: https://doi.org/10.1016/j.bbalip.2016.06.019 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CFTR |
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| | Mucins |
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| | Mucosal barrier |
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| | Mucus |
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| | Phosphatidylcholine |
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| | Tight junctions |
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| | Ulcerative colitis |
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| K10plus-PPN: | 156981631X |
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| Verknüpfungen: | → Zeitschrift |
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Phosphatidylcholine passes through lateral tight junctions for paracellular transport to the apical side of the polarized intestinal tumor cell-line CaCo2 / Stremmel, Wolfgang [VerfasserIn]; September 2016 (Online-Ressource)
